PMID: 2115553Jul 3, 1990Paper

A rapid one step purification procedure for murine IgD based on the specific affinity of Bandeiraea (Griffonia) simplicifolia-1 for N-linked carbohydrates on IgD

Journal of Immunological Methods
J D OppenheimG J Thorbecke

Abstract

The alpha-D-galactopyranosyl binding lectin from the seeds of Bandeiraea simplicifolia (a.k.a. Griffonia simplicifolia) termed BS-I, strongly reacts with murine IgD and with no other protein in ascites including all other classes of immunoglobulins as determined by immunoprecipitation, hemagglutination inhibition and affinity binding. Based on this finding, murine IgD could be rapidly purified directly from whole ascitic fluid by passage over affinity beads of BS-I linked to Sepharose 4B and subsequent elution by a buffer containing 0.1 M D-galactose. The sugar eluted product is 95-99% pure as determined by SDS-PAGE and represents 90-95% of the total IgD in the initial ascites by ELISA assay. Both monomeric and dimeric murine IgD may be purified by this procedure. Human IgD is unreactive with this lectin. Treatment of purified IgD with endoglycosidases that remove either O- or N-linked glycosides indicates that BS-I binds to IgD only via N-linked carbohydrate chains.

References

Jan 1, 1976·Scandinavian Journal of Immunology·E Saltvedt
Jan 1, 1975·Scandinavian Journal of Immunology·E SaltvedtS Olsnes
Jan 1, 1990·Research in Immunology·A R AminG J Thorbecke
May 1, 1990·The Journal of Experimental Zoology·R Colon-Urban, J D Oppenheim
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S HabaA Nisonoff
Jun 1, 1989·Journal of Autoimmunity·R F van VollenhovenG W Siskind
Oct 1, 1988·Immunological Reviews·R F CoicoG J Thorbecke
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R F CoicoG J Thorbecke
May 1, 1987·Molecular Immunology·P AucouturierJ L Preud'homme
Jul 1, 1986·Scandinavian Journal of Immunology·R Marches, V Ghetie
Jan 1, 1984·The Journal of Experimental Medicine·B XueG J Thorbecke
Oct 1, 1982·European Journal of Immunology·R G Vasilov, H L Ploegh
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M S Neuberger, K Rajewsky

❮ Previous
Next ❯

Citations

Jan 1, 1990·Research in Immunology·A R AminG J Thorbecke
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S M TammaR F Coico
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A R AminG J Thorbecke
Feb 26, 1998·Immunological Reviews·C D Swenson, G J Thorbecke
May 30, 2003·Journal of Leukocyte Biology·Seetha M Lakshmi Tamma, Richard F Coico
Apr 7, 2009·Current Protocols in Immunology·Sarah M AndrewRichard Coico
Dec 29, 1995·Annals of the New York Academy of Sciences·C D SwensonG J Thorbecke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.