PMID: 7538479May 1, 1995Paper

A rapid permeabilization procedure for accurate quantitative determination of beta-galactosidase activity in yeast cells

FEMS Microbiology Letters
F Kippert

Abstract

A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or vortexing. This procedure is equally applicable to fresh and frozen samples. It is compared to earlier reported methods and found to be superior by being more accurate and less time-consuming.

References

Oct 1, 1978·Analytical Biochemistry·G F MiozzariR Hütter
May 1, 1987·Molecular and Cellular Biology·M R Slater, E A Craig
May 2, 1973·European Journal of Biochemistry·R SerranoC Gancedo
Mar 1, 1982·Analytical Biochemistry·H Felix
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Guarente, M Ptashne

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Citations

Apr 17, 2008·Journal of Industrial Microbiology & Biotechnology·Jessy Abraham, S G Bhat
Mar 1, 2012·Journal of Industrial Microbiology & Biotechnology·S GogniesA Belarbi
Jul 28, 2013·Antonie van Leeuwenhoek·Joanna BerlowskaWojciech Ambroziak
Nov 10, 2011·The Journal of Microbiology·Ji-Hyeon Choi, Sang-Hyun Park
Mar 8, 2005·Fungal Genetics and Biology : FG & B·Agnieszka M JaniakJeffrey M Becker
Nov 8, 2011·Journal of Microbiological Methods·Lekha Menon MargasseryJohn P Morrissey
Jun 11, 2002·Journal of Molecular Biology·Vaea Richard de SoultraitBernard Roques
May 22, 2002·Biochimica Et Biophysica Acta·Vaea Richard de SoultraitMichel Fournier
Sep 23, 2000·Molecular and Cellular Endocrinology·V Panet-RaymondM A Trifiro
Mar 7, 2002·Toxicology Letters·Marc B Cox, Charles A Miller
Nov 21, 1998·Molecular Microbiology·V DenisB Daignan-Fornier
Feb 7, 2014·Applied Biochemistry and Biotechnology·Jay Shankar Singh YadavR Y Surampalli
Nov 2, 2001·Molecular and Cellular Biology·K RéboraB Daignan-Fornier
Jul 24, 2004·Cell Stress & Chaperones·Marc B Cox, Charles A Miller
Jul 29, 2009·Genetics·Christelle Saint-MarcBertrand Daignan-Fornier
Nov 10, 2009·The Journal of Biological Chemistry·Cyrille ChevtzoffAnne Devin
Oct 13, 2006·FEMS Yeast Research·Martin ClémentPierre Belhumeur
Feb 26, 2016·Molecular Biology of the Cell·Naveen Kumar Chandappa GowdaClaes Andréasson
Nov 24, 2004·Molecular Microbiology·Deborah A HoganRoberto Kolter
Aug 5, 2000·The Journal of Peptide Research : Official Journal of the American Peptide Society·S LiuF Naider

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