A rapid pneumococcal serotyping system based on monoclonal antibodies and PCR

Journal of Medical Microbiology
Jigui YuM H Nahm

Abstract

Streptococcus pneumoniae expresses at least 91 different polysaccharide (PS) capsules and the currently available serotyping methods are tedious to perform. We have been developing a rapid pneumococcal serotyping assay (named the 'multibead assay') based on the capacity of pneumococcal lysates to inhibit the ability of 24 different anti-capsule antibodies to bind to latex beads coated with 24 different PSs (serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9N, 9V, 14, 18C, 19A, 19F, 23F, 2, 8, 10A, 11A, 12F, 15B, 17F, 20, 22F and 33F). Because the polyclonal antibodies used for 10 serotypes (2, 8, 10A, 11A, 12F, 15B, 17F, 20, 22F and 33F) had limited serotype specificity, we replaced them with monoclonal antibodies for the 10 serotypes. To extend the serotype coverage beyond the 24 serotypes, we have adapted multiplexed PCR for five additional serotypes (15A, 15C, 16F, 35B and 38) to be useful with the pneumococcal lysates prepared for the multibead assay. We then validated the combined assay with 157 clinical isolates from the Centers for Disease Control and Prevention and found that the new combined assay produced results that are concordant with the quellung reaction. The combined assay is robust and could be used to rapidly identify the se...Continue Reading

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Citations

Nov 16, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I VickersH Humphreys
May 29, 2010·The Journal of Infectious Diseases·Juan J Calix, Moon H Nahm
Jan 24, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Juan J CalixMoon H Nahm
Nov 26, 2009·The Pediatric Infectious Disease Journal·Caritas M FrancoMaria-Cristina de Cunto Brandileone
Sep 9, 2011·Clinical and Vaccine Immunology : CVI·Jigui YuMoon H Nahm
Jan 14, 2009·Infection and Immunity·Vishakha SabharwalStephen I Pelton
Dec 3, 2010·Journal of Clinical Microbiology·Jigui YuMoon H Nahm
Sep 3, 2009·Future Microbiology·Mary Catherine McEllistrem
Aug 21, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M Catherine McEllistrem, Moon H Nahm
Dec 24, 2013·Microbes and Infection·Vishakha SabharwalStephen I Pelton
Jun 12, 2013·Infection·H ZhaoY J Juhn
Jan 23, 2010·Journal of Microbiological Methods·J M MarimonE Perez-Trallero
Apr 17, 2009·Journal of Medical Microbiology·Alexander ThürmerPaul Christian Lück
Apr 7, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G VernetK P Klugman
Aug 24, 2011·Journal of Korean Medical Science·Ki Wook YunHoan Jong Lee
Sep 24, 2010·Postgraduate Medicine·Ji A JungYoung J Juhn

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