PMID: 6987495Jan 1, 1980Paper

A rapid procedure for measurement of DNA repair in human fibroblasts and for complementation analysis of xeroderma pigmentosum cells

Mutation Research
A R Lehmann, S Stevens

Abstract

A rapid procedure for measuring unscheduled DNA synthesis has been studied in detail. Human fibroblasts were brought into the non-dividing state by either growing to confluence or starvation for arginine. Residual semi-conservative synthesis was abolished by hydroxyurea. Hydroxyurea-resistant DNA synthesis which was induced by irradiation and chemical mutagens was presumed to represent repair synthesis and provided a very rapid semi-quantitative procedure for its measurement. Problems were encountered, however, when comparing the quantitative response of different cell strains. The variability between experiments was quite large, and we found that the level of repair synthesis depended not only on the mutagen and the genotype of the cell, but also on physiological factors. This led to some anomalous results. The system was able to detect with ease the large defects in UV-induced repair synthesis in fibroblasts from patients with xeroderma pigmentosum (XP) but it would probably not easily detect less than a 50% reduction in the level of repair synthesis. By extension of this procedure, in combination with cell fusion induced by polyethylene glycol, we have developed a method for carrying out genetic complementation of XP fibrobl...Continue Reading

References

May 19, 1976·Biochimica Et Biophysica Acta·C A Smith, Philip C Hanawalt
Oct 4, 1976·Biochimica Et Biophysica Acta·C A Smith, Philip C Hanawalt
Aug 1, 1979·Mutation Research·W KeijzerD Bootsma
Dec 1, 1978·South African Journal of Surgery. Suid-Afrikaanse Tydskrif Vir Chirurgie·F Kalk, T Jenkins
Mar 1, 1977·Mutation Research·A R CollinsR T Johnson
Jul 15, 1977·Journal of Molecular Biology·Y Fujiwara, M Tatsumi
Jun 15, 1976·Molecular and Cellular Biochemistry·S Kit
Dec 1, 1975·Mutation Research·K H KraemerD Bootsma
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Jul 19, 1972·Nature: New Biology·E A de Weerd-KasteleinD Bootsma
Apr 1, 1971·Photochemistry and Photobiology·E Ben-Hur, R Ben-Ishai
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Aug 1, 1964·Journal of Molecular Biology·D PETTIJOHN, Philip C Hanawalt

Citations

Jan 1, 1981·Human Genetics·P C Huang, R B Sheridan
Apr 1, 1984·Journal of the Neurological Sciences·S H Robison, W G Bradley
Mar 20, 1985·Biochimica Et Biophysica Acta·R C BirdB H Sells
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L H ThompsonD A Glaser
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·E M TaylorA R Lehmann
Jun 24, 2008·Brain : a Journal of Neurology·Anu AnttinenReijo J Marttila
Feb 25, 1982·Nucleic Acids Research·A R CollinsR T Johnson
Nov 3, 2011·Orphanet Journal of Rare Diseases·Alan R LehmannM Stefanini
Jun 10, 2014·The Journal of Clinical Investigation·Emma L BapleAndrew H Crosby
Feb 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hiva FassihiAlan R Lehmann
Jan 25, 2014·TheScientificWorldJournal·Subhash MareddyPreethi Balan
Jul 6, 2014·Scientific Reports·Tatsuya TogaShigenori Iwai
Jun 26, 2018·Pigment Cell & Melanoma Research·Zeinab KasraianHamid Reza Rezvani
Nov 8, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hiva Fassihi

Related Concepts

Arginine hydrochloride
Cell Fusion Procedure
DNA, Double-Stranded
Base Excision Repair
Cis-Trans Test
Genetic Techniques
Skin
Black Light, Ultraviolet
Xeroderma Pigmentosum

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