PMID: 11311349Apr 20, 2001Paper

A rapid real-time quantitative polymerase chain reaction for hepatitis B virus

Journal of Virological Methods
K BrechtbuehlN A Saunders

Abstract

Quantification of hepatitis B virus (HBV) DNA in serum is important for monitoring treatment. A rapid and cost effective alternative to the methods available currently was developed based on a real-time quantitative polymerase chain reaction (PCR) done in the LightCycler apparatus. Primers and a probe for sequences of the surface gene of HBV were designed and quantification achieved by reference to standards containing known concentrations of the target sequence. A single copy of the HBV genome could be detected if present in the reaction mixture. The quantitative range of the assay was from 4 x 10(2) to 1.3 x 10(10) surface gene copies/ml serum. Nested PCR was required for quantification in the lower part of this range (<10(5) copies). The real-time PCR and Amplicor Monitor (Roche) tests performed comparably at virus concentrations below 10(6) copies/ml. The commercial test underestimated higher concentrations of virus.

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Citations

May 6, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sani Hussein AliyuMartin David Curran
May 15, 2002·Journal of Virological Methods·D ParaskevisA Hatzakis
May 3, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stephan SchaeferWolfram H Gerlich
Oct 17, 2002·Trends in Parasitology·Andrew S Bell, Lisa C Ranford-Cartwright
Mar 9, 2002·Nucleic Acids Research·Ian M MackayAndreas Nitsche
Aug 17, 2002·Antimicrobial Agents and Chemotherapy·Růzena StránskáRob Schuurman
Mar 5, 2005·Applied and Environmental Microbiology·Chang-Ping YuKung-Hui Chu
Jan 19, 2006·Clinical Microbiology Reviews·M J EspyT F Smith
Sep 15, 2004·Journal of Clinical Microbiology·Danny Ka-Ho WongChing-Lung Lai
Oct 3, 2002·American Journal of Respiratory and Critical Care Medicine·Hajime FukunagaTokuhiro Ishihara
Jan 6, 2012·Virology Journal·Jijing TianJun Yin
Nov 18, 2005·Biosensors & Bioelectronics·Yoon-Kyoung ChoChristopher Ko
Jun 25, 2002·Expert Opinion on Biological Therapy·Monica C PanelliFrancesco M Marincola
Apr 3, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Lili GuoTao Duan
Dec 25, 2007·The Korean journal of laboratory medicine·Sang Hyun HwangHeung Bum Oh
Mar 11, 2004·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I M Mackay
May 2, 2003·Journal of Medical Microbiology·Stephen K N HoTak-Mao Chan
Mar 20, 2015·Jundishapur Journal of Microbiology·Payam BehzadiSeyed Moayed Alavian
May 3, 2002·Analytical Chemistry·Matthew M FerrisKathy L Rowlen
Feb 22, 2003·Journal of the American Society of Nephrology : JASN·Hitomi KawanoHiroshi Iwao

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