A rapid RNA extraction method from oil palm tissues suitable for reverse transcription quantitative real-time PCR (RT-qPCR)

3 Biotech
Siti Suriawati BadaiMat Yunus Abdul Masani

Abstract

Cetyltrimethylammonium bromide (CTAB) is the preferred detergent in RNA extraction of oil palm tissues. However, the CTAB-based protocol is time-consuming. In this study, a combination of the CTAB-based method and silica-based purification reduced the extraction time from two days to five hours. Quality of total RNA from 27 different tissues of oil palm was shown to have an RNA integrity number (RIN) value of more than seven. The extracted RNA was evaluated by RT-qPCR using three reference oil palm genes (GRAS, CYP2, and SLU7) and three putative mesocarp-specific transcripts annotated as WRKY DNA-binding protein 70 (WRKY-70), metallothionein (MT) and pentatricopeptide repeat (PPR) genes. Tissue-specific expression profiling across complete developmental stages of mesocarp and vegetative tissues was determined in this study. Overall, the RNA extraction protocol described here is rapid, simple and yields good quality RNAs from oil palm tissues.

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 27, 2002·Food and Nutrition Bulletin·A S H Ong, S H Goh
Jun 5, 2002·The Plant Cell·Gerard J Bishop, Csaba Koncz
Mar 26, 2003·Analytical Biochemistry·Arun Dev SharmaPrabhjeet Singh
Oct 6, 2004·Preparative Biochemistry & Biotechnology·Zhihua LiaoKexuan Tang
Aug 3, 2005·BioTechniques·Marisa L Wong, Juan F Medrano
Feb 2, 2006·BMC Molecular Biology·Andreas SchroederThomas Ragg
Feb 14, 2006·Molecular Aspects of Medicine·Simone Fleige, Michael W Pfaffl
Feb 15, 2011·Nucleic Acids Research·Joëlle VermeulenJo Vandesompele
Oct 1, 2013·Journal of Applied Genetics·Bartłomiej Kozera, Marcin Rapacz
Jan 30, 2014·Annual Review of Plant Biology·Alice Barkan, Ian Small
Dec 7, 2014·Plant Cell Reports·Ghulam Kadir Ahmad ParveezRavigadevi Sambanthamurthi
Mar 26, 2016·Frontiers in Plant Science·Jose V DieLisa J Rowland
Apr 14, 2016·BioTechniques·Jessica DaviesJames Hadfield
May 21, 2016·Wiley Interdisciplinary Reviews. RNA·Radmila HrdlickovaBin Tian
Sep 13, 2016·Chemical Research in Toxicology·Judy L Bolton, Tareisha Dunlap
Jan 12, 2017·Biophysical Journal·Andrea M KatzLois Pollack
Aug 18, 2017·The Plant Cell·Junyi ChenThomas Dresselhaus
Jun 21, 2018·Biomolecular Detection and Quantification·Bhaja K PadhiSabit Cakmak
Jan 17, 2019·Bioscience Reports·Yimiao XiaBoye Liu
Jan 19, 2019·Trends in Biotechnology·Sean C TaylorJoshua Fenrich

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