PMID: 6984450Dec 17, 1982Paper

A rapid rosette technique for quantitation and separation of mononuclear cell subsets using monoclonal antibodies

Journal of Immunological Methods
T Egeland, T Lea

Abstract

We present a rapid and simple method for simultaneous quantitation and separation of mononuclear cell (MNC) subsets. When lymphoid cells are sensitized with monoclonal antibodies of the OK and Leu series, they rapidly form rosettes with ox erythrocytes (ORBC) coated with affinity-purified rabbit IgG against mouse IgG. Rosette-forming cells (RFC) may then be counted and separated from non-rosetting MNC by Isopaque-Ficoll gradient centrifugation. The yield and viability are close to 100% after ORBC lysis. Adherent cells do not interfere. Isolated T8+ and Leu3a+ cells were further tested: the purity was 97-99%, and the cells were functionally intact with respect to their modulating activity on the generation of immunoglobulin-secreting cells by MNC after stimulation with pokeweed mitogen.

References

Jan 1, 1978·Journal of Immunological Methods·J W Goding
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T W ChangG Goldstein
Sep 30, 1982·Journal of Immunological Methods·T EgelandO J Mellbye
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A HoffmanG Goldstein
Jan 1, 1981·Journal of Immunological Methods·S FongJ H Vaughan

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Citations

Sep 1, 1990·Scandinavian Journal of Immunology·L ThorsteinssonO Jensson
Jan 1, 1988·Clinical Immunology and Immunopathology·L D NotarangeloA Albertini
Jan 1, 1984·Pathology·R K Kumar, A W Lykke
Oct 1, 1983·Scandinavian Journal of Immunology·T EgelandO J Mellbye
Mar 1, 1984·Scandinavian Journal of Immunology·S BjerckeE Thorsby
Jan 1, 1987·Scandinavian Journal of Immunology·T Egeland, H Lund
Jan 1, 1990·European Journal of Haematology·J HelgestadT Hovig

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