A rapid Salmonella detection method involving thermophilic helicase-dependent amplification and a lateral flow assay

Molecular and Cellular Probes
Xin-Jun DuShuo Wang

Abstract

Salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. Since conventional culture methods to detect Salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. In this study, we developed a rapid, simple and portable Salmonella detection strategy that combines thermophilic helicase-dependent amplification (tHDA) with a lateral flow assay to provide a detection result based on visual signals within 90 min. Performance analyses indicated that the method had detection limits for DNA and pure cultured bacteria of 73.4-80.7 fg and 35-40 CFU, respectively. Specificity analyses showed no cross reactions with Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Enterobacter aerogenes, Shigella and Campylobacter jejuni. The results for detection in real food samples showed that 1.3-1.9 CFU/g or 1.3-1.9 CFU/mL of Salmonella in contaminated chicken products and infant nutritional cereal could be detected after 2 h of enrichment. The same amount of Salmonella in contaminated milk could be detected after 4 h of enrichment. This tHDA-strip can be ...Continue Reading

Citations

Sep 19, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Dakrong PissuwanMichael B Cortie
Sep 22, 2017·Analytical and Bioanalytical Chemistry·Susana Barreda-GarcíaMaría Jesús Lobo-Castañón
Jun 28, 2018·Analytical and Bioanalytical Chemistry·Sherine F CheungDaniel T Kamei
Feb 8, 2019·Journal of Food Science and Technology·Dilek Çam, Hüseyin Avni Öktem
Oct 6, 2020·SLAS Technology·Veronica R CampbellDaniel T Kamei

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