PMID: 6413851Oct 1, 1983Paper

A rapid screen for cumulative chromosomal damage in mice. Accumulation of circulating micronucleated erythrocytes

Mutation Research
R Schlegel, J T MacGregor

Abstract

The frequency of micronuclei in normochromatic peripheral blood erythrocytes was found to be a useful index of cumulative chromosomal damage in repeatedly exposed mice. Although approximately 5 weeks of continuous treatment is required to reach the maximum steady state level, significant elevations in this index were achieved after only 5 daily exposures to non-lethal doses of all six genotoxins tested. The frequencies of micronucleated cells per 1000 normochromatic erythrocytes in weanling mice after 5 daily intraperitoneal (i.p.) injections of each test agent were: triethylenemelamine (0.5 mg/kg), 13.7; mitomycin C (2 mg/kg), 7.1; cyclophosphamide (22 mg/kg), 6.6; colchicine (1 mg/kg), 4.0; nitrogen mustard (0.4 mg/kg), 3.6; 7,12-dimethylbenz[a]anthracene (5 mg/kg), 6.6. Controls averaged 1.2 per 1000. During extended exposure to nitrogen mustard (5 i.p. injections of 0.4 mg/kg per week), the incidence of micronucleated erythrocytes rose steadily to a value of 12.0 per 1000, approaching the steady state after about 5 weeks of treatment. These results indicate that the measurement of micronuclei in peripheral blood erythrocytes is an effective and rapid method for estimating chromosomal damage in subchronically or chronically ...Continue Reading

Citations

Mar 1, 1988·Stain Technology·R K Das, B Roy
Jul 3, 1998·International Journal of Cancer. Journal International Du Cancer·R M Balansky, S De Flora
Aug 4, 2015·Biological & Pharmaceutical Bulletin·Eduardo Madrigal-BujaidarJosé A Morales-González
Mar 16, 2006·Environmental and Molecular Mutagenesis·Ion UdroiuCaterina Tanzarella
Dec 20, 2020·Mutation Research. Reviews in Mutation Research·Richard J Albertini, Debra A Kaden
Sep 1, 1988·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G P Meshram, K M Rao

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