A rapid, sensitive method for the quantitation of N-acetyl-S-(2-hydroxyethyl)-L-cysteine in human urine using isotope-dilution HPLC-MS-MS

Journal of Analytical Toxicology
D B Barr, David L Ashley

Abstract

Because of increasing concern about exposure to carcinogens and other toxicants, reliable methods for biological monitoring of potentially exposed populations must be developed. For biological monitoring to be useful, appropriate biomarkers of exposures to xenobiotics must be identified, and sensitive, specific methods for quantifying the targeted biomarker must be developed. We have developed a sensitive and selective method for the analysis of N-acetyl-S-(2-hydroxyethyl)-L-cysteine (HEMA), urinary metabolite of at least three different known human carcinogens (vinyl chloride, ethylene oxide, and ethylene dibromide). The method uses strong anion-exchange solid-phase extraction and isotope-dilution high-performance liquid chromatography-tandem mass spectrometry. Our method is simple and is not labor intensive; the preparation time per sample is less than 10 min. Because urine samples vary in both their concentration and ion strength, intersample variability in HEMA recovery during the extraction is large. To overcome this inherent limitation, we use the isotope-dilution technique, which allows a complete correction for the extraction recovery for each sample. The limit of detection of the method is 0.68 microgram/L in a 1-mL ur...Continue Reading

Citations

Mar 14, 2003·Environmental Health Perspectives·Dana B BarrEric J Sampson
Mar 26, 2009·Chemical Research in Toxicology·Steven G CarmellaStephen S Hecht
Oct 31, 2000·Critical Reviews in Toxicology·R Thier, H M Bolt
Mar 17, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gerhard SchererHans-Juergen Roethig
Apr 30, 2020·Environmental Health Perspectives·Dana Boyd BarrUNKNOWN (HAPIN Investigative Team)
Apr 10, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Vincent HaufroidPhilippe Hotz

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