A rat brain protein expression map including cytosolic and enriched mitochondrial and microsomal fractions

Electrophoresis
Kurt KrapfenbauerGert Lubec

Abstract

Proteomics is a powerful tool to screen brain protein expression but the methodology is hampered by low abundance of proteins or compartmentalization or overload of high-abundance proteins. It was therefore the aim of the study to determine the expression of brain proteins by using enriched cellular subfractions and pre-electrophoretic chromatographical separation of brain homogenates. We used two-dimensional electrophoresis with subsequent matrix-assisted laser desorption/ionization (MALDI) detection and characterization of brain proteins. Subfractionation into cytosolic, mitochondrial and microsomal compartments was performed by ultracentrifugation. Pre-electrophoretic fractionation of the cytosolic fractions was carried out by ion exchange column chromatography. We detected and identified a large series of 437 proteins in rat brain and have shown proteins specific for the individual subcellular compartments. These proteins included housekeeping, signaling, cytoskeletal, intermediary metabolism, antioxidant proteins on the one and neuron and synaptosomal specific proteins on the other hand. Using fractionations of brain homogenates we were able to improve the power of the method on forming the basis for brain protein expressi...Continue Reading

Citations

Jan 20, 2005·Electrophoresis·Pier Giorgio RighettiEgisto Boschetti
Nov 12, 2010·Amino Acids·Yanwei SunGert Lubec
Jul 2, 2005·Journal of Chromatography. a·Spyros GarbisMichael Fountoulakis
Mar 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michael FountoulakisGert Lubec
Mar 1, 2005·Journal of Molecular and Cellular Cardiology·Michael FountoulakisYassemi Capetanaki
Jun 5, 2004·Neurochemical Research·Willard M Freeman, Scott E Hemby
Feb 3, 2004·Proteome Science·Joo-Ho ShinGert Lubec
Sep 22, 2012·BMC Research Notes·Ivan DimauroMalcolm J Jackson
Jul 8, 2008·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ying ZhangGuang-wei Zhong
Apr 20, 2011·Expert Review of Proteomics·Joern Barth, Walter Volknandt
Feb 12, 2005·Electrophoresis·George TsangarisMichael Fountoulakis
Nov 21, 2009·Drug and Alcohol Dependence·Melinda E LullKent E Vrana
Apr 27, 2005·Proteomics·Frank A WitzmannJay R Simon
Jun 29, 2005·Proteomics·Sabine P SchrimpfPeter Sonderegger
Apr 4, 2009·Proteomics·Devendra Kumar MauryaPurnima Bhargava
Mar 24, 2006·Electrophoresis·Michael Fountoulakis, Sophia Kossida
Mar 16, 2007·Journal of Neurochemistry·Jacqueline Burré, Walter Volknandt
Aug 24, 2006·Proteomics·Young Mok ParkJong Shin Yoo
Nov 1, 2007·Proteomics. Clinical Applications·Ming D Li, Ju Wang
Feb 25, 2006·Mass Spectrometry Reviews·Andrew K OttensNancy D Denslow
Nov 9, 2010·Proteomics·Devendra Kumar MauryaPurnima Bhargava
Oct 17, 2009·Proteomics·Purnima Bhargava
Mar 26, 2005·Brain Research. Molecular Brain Research·Jinghua JinJing Zhang
Oct 7, 2004·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C M Morris, K E Wilson
Sep 15, 2004·Brain Research. Molecular Brain Research·Oscar AlzateKevin V Hackshaw
Oct 7, 2004·European Journal of Pharmacology·Freya G G VercauterenRémi Quirion
Jul 6, 2004·Journal of Chromatography. a·Michael FountoulakisGert Lubec
Apr 4, 2006·Neurochemistry International·Anna Bierczynska-KrzysikJerzy Silberring
Mar 13, 2014·Journal of Neuroscience Methods·Rachel Yoon Kyung ChangAmanda Nouwens
Oct 19, 2006·Biochimica Et Biophysica Acta·Anu JalankoLeena Peltonen
Oct 16, 2004·International Review of Neurobiology·Frank A Witzmann, Wendy N Strother

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