A rat model for sickle cell-mediated vaso-occlusion in retina

Microvascular Research
G A LuttyR L Nagel

Abstract

Occlusions of the retinal vasculature are the initiating event in sickle cell retinopathy. In order to understand the mechanism(s) of sickle cell-mediated occlusion, a rat model was developed. Red blood cells (RBCs) from patients homozygous for hemoglobin (Hb) S (SS) or double heterozygous for Hb S and Hb C (SC) were separated on Percoll-Larex continuous density gradients, labeled with fluorescein isothiocyanate (FITC), and delivered via the left ventricle to anesthetized, ventilated rats. Blood gas levels were altered by changing inspired gas and monitored via a femoral arterial catheter. After the RBCs circulated for 5 min, animals were perfused with heparinized saline, the eyes enucleated, and the retinas removed and processed by our ADPase flatmount technique. The retinal vasculature was visualized under dark-field illumination and the FITC-RBCs visualized by fluorescence microscopy. Greater numbers of high-density SS cells (SS4, which consist of dense, dehydrated discocytes and irreversible sickled cells) were retained in the normal rat retinal vasculature than normal-density SS cells (SS2, which have the same density as normal AA cells, but consist of reticulocytes and young cells). Retention of SS4 cells was inversely de...Continue Reading

Citations

May 19, 2007·Lymphatic Research and Biology·E I GalanzhaV P Zharov
May 30, 2002·The British Journal of Ophthalmology·M Kunz MathewsG A Lutty
May 11, 2010·Survey of Ophthalmology·Mohammed ElagouzSobha Sivaprasad
Oct 25, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D Scott McLeodGerard Lutty
Dec 11, 2008·Experimental Eye Research·Stephen F OsterGerard A Lutty
Jul 25, 2017·Journal of Pediatric Hematology/oncology·Daniel A PahlRoyce W S Chen
Feb 27, 2001·Current Opinion in Hematology·R L Nagel
Dec 16, 2000·The Journal of Comparative Neurology·K R Zahs, T Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.