A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation

Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
Wei ZhangLiang-Duo Jiang

Abstract

To study the approach for developing a rat model of pulmonary fibrosis induced by bleomycin (BLM). Different doses (7, 6, 5, 3.4, 2, 1 mg/kg) of bleomycin A5-saline were infused into the rats' lung in bleomycin-treated group through tracheal intubation, and rats in sham-operated group were infused with same volume of saline. The living state and lung pathology of the rats were observed. The author deeply studied the condition of the rats in 1 mg/kg bleomycin-treated group, and the changes of body weight and lung pathology were observed. Lung quotient, the content of transforming growth factor beta1(TGF-beta1) and platelet-derived growth factor (PDGF) in serum were measured on the 14th, 28th and 45th day of the experiment. The study demonstrated that infusing large doses of bleomycin A5 quickly through tracheal intubation had a high mortality, and infusing 1 mg/kg quickly could successfully develop an animal model of pulmonary fibrosis. Compared with the sham-operated group, fibrosis was appeared obviously in the rats' lung in 1 mg/kg bleomycin A5-treated group after 14 days of experiment, diffuse fibrosis was appeared after 28 days of experiment, and the fibrosis became more severe after 45 days of experiment. The body weight o...Continue Reading

Citations

Oct 3, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Lin-Feng YanGuang-Bin Cui
Jun 19, 2008·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Wei ZhangXiang-feng Lu
Apr 3, 2012·Chinese Journal of Integrative Medicine·Chuan-Jian LuJing-Wen Deng

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