A ratiometric fluorescent biosensor for the sensitive determination of α-glucosidase activity and acarbose based on N-doped carbon dots

The Analyst
Mengjun WangXingguang Su

Abstract

In this work, a novel ratiometric fluorescent platform for α-glucosidase (α-glu) and its inhibitor was constructed based on N-doped carbon dots (N-CDs). The α-glucosidase present can catalyze the release of hydroquinone (HQ) from α-arbutin. Then, the generated HQ can be oxidized and copolymerized with polyethyleneimine (PEI) to form a yellowish green fluorescence copolymer (PHQ-PEI) with intense fluorescence emission at 510 nm. When the PHQ-PEI was formed, blue fluorescence of N-CDs at 425 nm was decreased, whereas the fluorescence of PHQ-PEI at 510 nm increased sharply as a result of the fluorescence resonance energy transfer (FRET) effect between N-CDs and PHQ-PEI. However, in the presence of acarbose, the activity of α-glucosidase is inhibited, and α-arbutin cannot be hydrolyzed to hydroquinone, leading to the fluorescence recovery of N-CDs at 425 nm and the fluorescence decrease of PHQ-PEI at 510 nm. The linear range from 0.2 to 1.6 mU mL-1 and 25-150 μmol L-1 was obtained for α-glucosidase and acarbose detection, respectively, and the detection limit (LOD) for α-glucosidase and acarbose was as low as 0.082 mU mL-1 and 14.5 μmol L-1. Thus, a ratiometric fluorescent sensor with good sensitivity and high specificity was estab...Continue Reading

References

Jul 1, 2009·Diabetes Care·Abbas E KitabchiJoseph N Fisher
Aug 6, 2010·Angewandte Chemie·Sheila N Baker, Gary A Baker
Jun 6, 2014·Nature·Rachel J PerryGerald I Shulman
Apr 8, 2017·ACS Applied Materials & Interfaces·Yueh-Yun YaoJia-Yaw Chang
Feb 20, 2019·Small·Jianjun DuXiaojun Peng

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Methods Mentioned

BETA
biosensing
fluorescence
transmission electron microscopy
FRET
biosensor
fluorescence sensing

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