A Rationale for the Use of Clotted Vertebral Bone Marrow to Aid Tissue Regeneration Following Spinal Surgery.

Scientific Reports
Francesca SalamannaMilena Fini

Abstract

Vertebral body bone marrow aspirate (V-BMA), easily accessible simultaneously with the preparation of the site for pedicle screw insertion during spinal procedures, is becoming an increasingly used cell therapy approach in spinal surgery. However, the main drawbacks for V-BMA use are the lack of a standardized procedure and of a structural texture with the possibility of diffusion away from the implant site. The aim of this study was to evaluate, characterize and compare the biological characteristics of MSCs from clotted V-BMA and MSCs from whole and concentrate V-BMAs. MSCs from clotted V-BMA showed the highest cell viability and growth factors expression (TGF-β, VEGF-A, FGF2), the greatest colony forming unit (CFU) potency, cellular homogeneity, ability to differentiate towards the osteogenic (COL1AI, TNFRSF11B, BGLAP) and chondrogenic phenotype (SOX9) and the lowest ability to differentiate toward the adipogenic lineage (ADIPOQ) in comparison to all the other culture conditions. Additionally, results revealed that MSCs, differently isolated, expressed different level of HOX and TALE signatures and that PBX1 and MEIS3 were down-regulated in MSCs from clotted V-BMA in comparison to concentrated one. The study demonstrated for...Continue Reading

References

Feb 11, 1993·Nucleic Acids Research·E M WrightP Koopman
Oct 19, 2001·Biochemical and Biophysical Research Communications·S TsutsumiY Kato
Jan 30, 2002·Acta Haematologica·N Ferrara, H P Gerber
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 24, 2002·Experimental Hematology·Nadia QuiriciGiorgio Lambertenghi Deliliers
Feb 5, 2003·Clinical Orthopaedics and Related Research·George F MuschlerKirk Easley
Jun 6, 2003·British Journal of Haematology·Barbara J Bain
Mar 23, 2004·Journal of Anatomy·Matthias SchiekerStefan Milz
Apr 6, 2004·Journal of Clinical Laboratory Analysis·Roger S RileyJonathan M Ben-Ezra
Jun 24, 2005·The Journal of Bone and Joint Surgery. British Volume·P HernigouH Rouard
Mar 27, 2007·Injury·Robert BielbyDennis McGonagle
Sep 18, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Abigail WoodfinSussan Nourshargh
May 5, 2010·Molecular and Cellular Biology·Jonathan A R GordonJane B Lian
Jan 3, 2013·Current Pharmaceutical Design·Laura de Girolamo Italian Mesenchymal Stem Cell Group
Sep 26, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Giovanni Barbanti BrodanoMaria Cristina Magli
Nov 5, 2013·The Spine Journal : Official Journal of the North American Spine Society·Raghav BadrinathJonathan N Grauer
Feb 6, 2014·Stem Cells·Laura E SidneyAndrew Hopkinson
Jun 20, 2014·American Journal of Hematology·Bhavana KondaEllen W Friedman
Dec 24, 2014·Indian Journal of Anaesthesia·Sanjeev PaltaAnshu Palta
Jan 27, 2015·World Journal of Stem Cells·Joshua SchroederMeir Liebergall
Dec 28, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Danielle R Rux, Deneen M Wellik
May 17, 2017·Cold Spring Harbor Perspectives in Biology·Ingo GrafeYuji Mishina
Jun 5, 2017·The Orthopedic Clinics of North America·Patrick C Schottel, Stephen J Warner
Jan 1, 2016·The Turkish Journal of Pediatrics·Tuğçe Tural-KaraFatoş Yalçınkaya
Jun 24, 2017·Journal of Cellular Physiology·Francesca SalamannaMilena Fini

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
density gradient centrifugation
flow
fluorescence microscopy
PCR
Assay

Software Mentioned

FACSDiva
R
R Project for Statistical Computing
R Foundation for Statistical Computing
WRS2
Primer Blast

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.