A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis ) in Food Products

Foods
Amaya VelascoCarmen G Sotelo

Abstract

Cephalopods are very relevant food resources. The common cuttlefish (Sepia officinalis) is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authentication of S. officinalis in food products to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of S. officinalis showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify, or showed a significantly different Ct (p < 0.001). The calculated efficiency of the system was 101%, and the minimum DNA quantity detected was 10-4 ng. No cross-reactivity was detected with any other species, thus, the designed method differentiates S. officinalis from other species of the genus Sepia and other cephalopod species and works for fresh, frozen, grilled, cooked and canned samples of Sepia spp. The method has proved to be reliable and rapid, and it may prove to be a useful tool for the control of fraud in cuttlefish products.

References

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Apr 18, 2008·Briefings in Bioinformatics·Sudhir KumarKoichiro Tamura
May 11, 2010·Comptes rendus biologies·Eivind Andreas Baste UndheimBryan Grieg Fry
Jun 1, 2015·Toxicon : Official Journal of the International Society on Toxinology·Tassanee LuekasemsukSoraya Chaturongakul
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Jan 17, 2016·Molecular and Cellular Probes·Jian YeZhiyuan Dai

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Citations

Aug 26, 2020·Critical Reviews in Food Science and Nutrition·Telmo J R FernandesIsabel Mafra
Dec 2, 2020·Biosensors·Giuseppina TatulliPier Paolo Pompa

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Datasets Mentioned

BETA
MN977161
MN977173

Methods Mentioned

BETA
Assay
PCR

Software Mentioned

Chromas
BLAST
MEGA
Bioedit

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