A reassessment of the response of the bacterial ribosome to the frameshift stimulatory signal of the human immunodeficiency virus type 1

RNA
Mélissa LégerLéa Brakier-Gingras

Abstract

HIV-1 uses a programmed -1 ribosomal frameshift to produce the precursor of its enzymes. This frameshift occurs at a specific slippery sequence followed by a stimulatory signal, which was recently shown to be a two-stem helix, for which a three-purine bulge separates the upper and lower stems. In the present study, we investigated the response of the bacterial ribosome to this signal, using a translation system specialized for the expression of a firefly luciferase reporter. The HIV-1 frameshift region was inserted at the beginning of the coding sequence of the luciferase gene, such that its expression requires a -1 frameshift. Mutations that disrupt the upper or the lower stem of the frameshift stimulatory signal or replace the purine bulge with pyrimidines decreased the frameshift efficiency, whereas compensatory mutations that re-form both stems restored the frame-shift efficiency to near wild-type level. These mutations had the same effect in a eukaryotic translation system, which shows that the bacterial ribosome responds like the eukaryote ribosome to the HIV-1 frameshift stimulatory signal. Also, we observed, in contrast to a previous report, that a stop codon immediately 3' to the slippery sequence does not decrease the...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C TuJ A Bruenn
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J D DinmanR B Wickner
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·J D DinmanS W Peltz
Jan 29, 2000·Molecular and Cellular Biology·J D LopinskiJ A Bruenn
Oct 3, 2000·Nature·B T WimberlyV Ramakrishnan
Apr 3, 2001·Science·M M YusupovH F Noller
May 8, 2001·Science·J M OgleV Ramakrishnan
Jun 8, 2001·Annual Review of Biochemistry·M V Rodnina, W Wintermeyer
Aug 21, 2001·Cell·G Z YusupovaH F Noller
Mar 21, 2002·FEBS Letters·Harry F NollerJ H D Cate
Mar 23, 2002·Cell·V Ramakrishnan
Apr 12, 2002·Gene·Pavel V BaranovJohn F Atkins
Jul 20, 2002·Journal of Molecular Biology·François BélangerLéa Brakier-Gingras
Sep 10, 2002·Trends in Biochemical Sciences·Jason W HargerJonathan D Dinman
Oct 16, 2002·Nature Structural Biology·Holger StarkMarin van Heel
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J F AtkinsR F Gesteland
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·I Brierley, S Pennell
May 27, 2003·Trends in Biochemical Sciences·James M OgleV Ramakrishnan
Oct 21, 2003·Nature Structural Biology·Mikel ValleJoachim Frank
Jan 20, 2004·RNA·Pavel V BaranovJohn F Atkins
Feb 5, 2004·Molecular Cell·Kirill B Gromadski, Marina V Rodnina
Apr 22, 2004·Journal of Molecular Biology·François BélangerLéa Brakier-Gingras

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Citations

Nov 25, 2004·Nucleic Acids Research·Jonathan L Jacobs, Jonathan D Dinman
Aug 14, 2009·ACS Chemical Biology·Ryan J MarcheschiSamuel E Butcher
Oct 9, 2009·Nucleic Acids Research·Marie-Hélène MazauricDominique Fourmy
Jun 9, 2011·ACS Chemical Biology·Ryan J MarcheschiSamuel E Butcher
Mar 13, 2012·Expert Opinion on Therapeutic Targets·Léa Brakier-GingrasSamuel E Butcher
Feb 8, 2006·Trends in Genetics : TIG·Pavel V BaranovJohn F Atkins
Jun 2, 2005·Journal of Molecular Biology·David W Staple, Samuel E Butcher
Jul 16, 2008·Virus Research·David P Giedroc, Peter V Cornish
Sep 18, 2007·Journal of Molecular Biology·Ryan J MarcheschiSamuel E Butcher
Aug 6, 2013·Journal of Theoretical Biology·Ping Xie
Nov 7, 2013·Analytical Chemistry·Tamaki Endoh, Naoki Sugimoto
May 20, 2017·RNA·Dustin B RitchieMichael T Woodside
Jul 15, 2021·Computational and Structural Biotechnology Journal·Kai-Chun Chang, Jin-Der Wen

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