A receptor tyrosine kinase, Sky, and its ligand Gas 6 are expressed in gonads and support primordial germ cell growth or survival in culture

Developmental Biology
N MatsubaraY Matsui

Abstract

Although a number of growth factors and their receptors are involved in the proliferation and differentiation of primordial germ cells (PGCs), the only factor that has been shown to be active in vivo is Steel factor, a ligand for c-Kit. To identify new growth factor receptors that may be required for PGCs function in vivo, we used an reverse transcription-polymerase chain reaction-based strategy to screen for protein kinase genes expressed in PGC-derived embryonic germ cells. We report here that one such gene encoding the receptor tyrosine kinase, Sky, is expressed in both PGCs and their supporting cells in male genital ridges after 11.5 dpc. Interestingly, Sky expression was not detected in female genital ridges, although transcripts were detected in supporting cells in the developing ovary at later stages. Gas 6, a ligand for Sky, was also expressed in interstitial cells which surround Sky positive cells in genital ridges, and, in addition, it supported PGC growth or survival in culture. After birth, Sky expression in testis was restricted to Sertoli cells, and Gas 6 was detected around peritubular cells and Leydig cells. These results suggest that Gas 6-Sky signaling plays a role in PGC growth, sexual differentiation, and Se...Continue Reading

Citations

May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C G Print, K L Loveland
Feb 21, 2004·Reproduction, Fertility, and Development·David Pei-Cheng LinHan-Hsin Chang
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shoutian ZhuXu Wu
Apr 3, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Y Matsui
Aug 24, 1999·Developmental Biology·Y Morita, J L Tilly
Nov 2, 2016·International Journal of Molecular Sciences·Mattia BellanPier Paolo Sainaghi
Oct 23, 2019·Disease Markers·Giacomo TondoCristoforo Comi
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A M HealyH W Farber
Dec 7, 2018·Cancers·Sherri K SmartDouglas K Graham

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