Abstract
Modulation of actin dynamics through the N-WASp/Arp2/3 pathway is important in cell locomotion, membrane trafficking, and pathogen infection. Here, we demonstrate that Nck is essential for actin remodeling stimulated by phosphatidylinositol 4,5 bisphosphate (PI(4,5)P(2)) and, conversely, that PI(4,5)P(2) is necessary for localized actin polymerization induced by Nck in vivo. Nck knockdown or knockout suppressed actin comets induced by phosphatidylinositol 5-kinase (PIP5K), and PIP5K stimulated tyrosine phosphorylation of an Nck SH2 domain binding partner, suggesting that Nck couples phosphotyrosine- and phosphoinositide-dependent signals. We show that PI(4,5)P(2) and PIP5K are both enriched at actin comets induced by Nck aggregates and that formation of actin comets was strongly inhibited by coclustering with an inositol 5-phosphatase domain to decrease local PI(4,5)P(2) levels. The extent of Nck-induced actin polymerization was also modulated by PI(4,5)P(2)-sensitive N-WASp mutants. This study uncovers a strong reciprocal interdependence between Nck and PI(4,5)P(2) in promoting localized N-WASp-mediated actin polymerization in cells.
References
Apr 18, 1998·The Journal of Cell Biology·L MaM W Kirschner
Feb 18, 1999·Current Biology : CB·F FrischknechtM Way
Nov 30, 1999·Nature·F FrischknechtM Way
Mar 21, 2000·Nature·A S KimM K Rosen
Apr 4, 2000·Current Biology : CB·A L RozelleH L Yin
Jun 28, 2000·The Journal of Cell Biology·T LauxP Caroni
Jul 6, 2000·Nature Cell Biology·V MoreauM Way
Oct 29, 2000·Science·K E PrehodaW A Lim
May 8, 2001·The Journal of Biological Chemistry·R RohatgiB J Mayer
Sep 5, 2001·Nature Cell Biology·S GruenheidB B Finlay
Oct 5, 2001·Nature Cell Biology·S B SnapperF W Alt
Oct 19, 2001·Oncogene·W LiD T Woodley
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·James D OrthMark A McNiven
Mar 28, 2002·Science's STKE : Signal Transduction Knowledge Environment·Tamas Balla, Péter Várnai
Jul 31, 2002·The Journal of Biological Chemistry·Stefanie BeneschKlemens Rottner
Jun 17, 2003·Molecular and Cellular Biology·Friedhelm BladtTony Pawson
Jan 9, 2004·Current Biology : CB·Gonzalo M RiveraBruce J Mayer
Jan 28, 2004·Cell·Tony Pawson
Feb 6, 2004·The Journal of Cell Biology·Kenneth G CampelloneJohn M Leong
Jan 25, 2005·Molecular Cell·Venizelos PapayannopoulosWendell A Lim
Feb 3, 2005·The Journal of Cell Biology·Hideki YamaguchiJohn Condeelis
Jun 14, 2005·International Journal of Biological Sciences·Yong Rao
Apr 8, 2006·The Journal of Biological Chemistry·Christopher J GuerrieroOra A Weisz
Oct 13, 2006·Nature·Gilbert Di Paolo, Pietro De Camilli
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Tadaomi Takenawa, Shiro Suetsugu
Mar 17, 2007·Critical Reviews in Biochemistry and Molecular Biology·Jessica N HeckRichard A Anderson
Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Shandiz TehraniJohn A Cooper
Jul 9, 2008·The Journal of Cell Biology·Tsukasa OikawaTadaomi Takenawa
Jul 25, 2008·Nature·Nathan A SalleeWendell A Lim
Nov 11, 2008·Molecular Cell·Shae B PadrickMichael K Rosen
Citations
Feb 26, 2013·Nature Neuroscience·Laura TrovòCarlos G Dotti
Nov 9, 2010·The Journal of Biological Chemistry·Partha S MitraIda S Owens
Sep 20, 2012·Journal of Lipid Research·Akua N RoachGuangwei Du
May 23, 2012·The Journal of Cell Biology·Jonathon A DitlevBruce J Mayer
Nov 27, 2015·Molecular Biology of the Cell·Sofya BorinskayaBruce J Mayer
Aug 13, 2015·Cellular and Molecular Life Sciences : CMLS·Loretta TuostoRicciarda Galandrini
Jul 28, 2013·Bioarchitecture·Sankar P Chaki, Gonzalo M Rivera
Jan 21, 2015·The Journal of Cell Biology·Lawrence L LeClaireDiane L Barber
Nov 1, 2008·PLoS Pathogens·Kenneth G CampelloneJohn M Leong
Oct 15, 2013·Immunological Reviews·Omri MatalonMira Barda-Saad
Sep 14, 2013·Molecular & Cellular Proteomics : MCP·Chad D WallsZhong-Yin Zhang
Jul 12, 2017·Nature Reviews. Immunology·Pavel Tolar
Sep 29, 2017·Molecular Biology of the Cell·David C MorrisGonzalo M Rivera
Aug 17, 2017·ELife·Yidi SunDavid G Drubin
Feb 26, 2010·Nature Reviews. Immunology·Adrian J Thrasher, Siobhan O Burns
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yosuke SenjuPekka Lappalainen
Apr 12, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Yue SunRichard A Anderson
Dec 30, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michela MuscoliniLoretta Tuosto
Oct 23, 2019·Cancer Medicine·Sankar P ChakiGonzalo M Rivera
Aug 14, 2019·Immunological Reviews·Chenguang XuWanli Liu
Feb 28, 2013·Journal of Cell Science·Sankar P ChakiGonzalo M Rivera
Nov 29, 2013·Journal of Cell Science·Ashley C HumphriesMichael Way
Sep 24, 2020·Developmental Cell·Jennifer H KongRajat Rohatgi
Jun 29, 2010·Experimental Cell Research·Shruti Haralalka, Susan M Abmayr
Sep 18, 2012·Cell Host & Microbe·Ashley C HumphriesMichael Way