PMID: 7538431Feb 1, 1994Paper

A recombinant amino-terminal fragment of bactericidal/permeability increasing protein (rBPI23) inhibits soluble CD14-mediated lipopolysaccharide-induced endothelial adherence for human neutrophils

Shock
K HuangD M Fishwild

Abstract

Exposure of cultured human umbilical vein endothelial cells (HUVEC) to lipopolysaccharide (LPS) or interleukin 1 (IL-1) causes increased expression of adhesion molecules such as E-selectin and CD54 by HUVEC and consequently increased adherence of peripheral blood neutrophils. A recombinant aminoterminal fragment of bactericidal/permeability increasing protein (rBPI23) was shown to specifically block the LPS-induced adhesiveness of HUVEC for neutrophils. rBPI23 also prevented the LPS- but not IL-1 beta-induced upregulation on HUVEC of E-selectin and CD54. Furthermore, this inhibition was evident even when the endothelial cells were exposed to LPS for up to 1-2 h prior to rBPI23 addition. The inhibitory effects of an anti-CD14 monoclonal antibodies (mAb) were similar to those of rBPI23. Combination of the anti-CD14 mAb and rBPI23 resulted inhibition greater than either one used alone. These studies demonstrate that rBPI23 acts as a specific and potent inhibitor of soluble CD14-mediated LPS induction.

Citations

Mar 10, 1999·Liver Transplantation and Surgery : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·M J WiezerP A van Leeuwen
May 6, 1998·Protein Science : a Publication of the Protein Society·L J BeamerD Eisenberg
Dec 31, 2003·Developmental and Comparative Immunology·Jørgen StenvikTrond Ø Jørgensen T
Nov 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Marco M DominguesNuno C Santos
Oct 3, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michiko YamagataGeorge L King
Jun 7, 2017·Journal of Food Science·Kyoko AsamiMakoto Kanauchi
May 25, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kwong-Fai WongSheung-Tat Fan
Jul 8, 2020·Journal of Food Science·Hitomi SasakiMakoto Kanauchi
Apr 2, 1998·Current Opinion in Immunology·P Elsbach, J Weiss

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.