A recombinant antigen-based enzyme-linked immunosorbent assay (ELISA) for lungworm detection in seals

Parasites & Vectors
Sophia Arlena UlrichChristina Strube

Abstract

Pinnipeds are frequently infected by the lungworms Otostrongylus circumlitus and Parafilaroides gymnurus (Metastrongyloidea). Infections are frequently associated with secondary bacterial bronchopneumonia and are often lethal. To date, a reliable lungworm diagnosis in individual seals is only possible during necropsy as examination of faeces collected from resting places does not allow assignment to individuals. Therefore, a diagnostic tool for lungworm detection in living seals is desirable for monitoring health of seals in the wild and in captivity. Previously, an ELISA based on recombinant bovine lungworm major sperm protein (MSP) as diagnostic antigen was developed for lungworm diagnosis in cattle. In the present study, this test was adapted for detection of antibodies against lungworms in harbour (Phoca vitulina) and grey seals (Halichoerus grypus). Furthermore, sera of northern elephant seals (Mirounga angustirostris) were tested to evaluate whether the harbour/grey seal ELISA is suitable for this seal species as well. For ELISA evaluation, lungworm-positive and -negative sera of harbour and grey seals were analysed using horseradish peroxidase (HRP)-conjugated Protein A as secondary antibody. Optical density was measured...Continue Reading

References

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Apr 10, 2014·WormBook : the Online Review of C. Elegans Biology·Harold E Smith
Apr 11, 2014·Seminars in Cell & Developmental Biology·Ronald E Ellis, Gillian M Stanfield

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Citations

Mar 16, 2016·International Journal for Parasitology. Parasites and Wildlife·Sophia Arlena UlrichUrsula Siebert
Sep 19, 2017·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Julie D SheldonNicole I Stacy
Aug 5, 2020·Parasitology Research·Simone MorelliDonato Traversa
Dec 29, 2020·International Journal for Parasitology. Parasites and Wildlife·M F A van WijngaardenT J Tobias
Jan 26, 2021·International Journal for Parasitology. Parasites and Wildlife·Anja ReckendorfChristina Strube

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

MedCalc®

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