A recombinant, arginine-glycine-aspartic acid (RGD) motif from foot-and-mouth disease virus binds mammalian cells through vitronectin and, to a lower extent, fibronectin receptors

Gene
A VillaverdeC Coutelle

Abstract

The cell-binding abilities of a recombinant, RGD-containing peptide from foot-and-mouth disease virus (FMDV) have been characterized in HeLa and BHK cells. This peptide represents the aa sequence of the solvent-exposed G-H loop of protein VP1 which is involved in cell recognition and infection. The efficiency of the viral motif in promoting cell attachment and spreading is comparable to that shown by fibronectin or vitronectin. Cell binding is inhibited by a monoclonal antibody directed against a viral, RGD-involving B-cell epitope and also by sera against vitronectin (alpha V beta 3/beta 5) and fibronectin (alpha 5 beta 1) receptors. In addition, a synthetic RGD peptide, which is a ligand for both integrins, prevents the cell binding mediated by the FMDV domain. These data demonstrate that the FMDV RGD motif is a potent ligand for cell-receptor integrins and sufficient to promote cell attachment to susceptible cells mainly through the vitronectin receptor.

References

Dec 15, 1992·European Journal of Biochemistry·M GurrathR Timpl
Mar 27, 1992·Science·J M BergelsonR W Finberg
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M G MateuE Domingo
Jun 1, 1985·The Journal of Cell Biology·E G HaymanE Ruoslahti
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E F PlowM H Ginsberg
Jun 30, 1994·Nature·R H JacobsonB W Matthews

❮ Previous
Next ❯

Citations

Dec 1, 2000·Biochemical and Biophysical Research Communications·A Arís, A Villaverde
Jul 11, 2001·Biochemical and Biophysical Research Communications·P AlcaláA Villaverde
May 3, 2003·Biochemical and Biophysical Research Communications·Anna Arís, Antonio Villaverde
Jun 4, 1998·Thrombosis Research·K T Preissner, D Seiffert
Jan 16, 2003·Virus Research·Terry JacksonElizabeth Fry
Sep 17, 1998·FEBS Letters·J X Feliu, A Villaverde
Apr 20, 2010·Advanced Drug Delivery Reviews·Brenda F Canine, Arash Hatefi
Aug 28, 2007·Annals of Neurology·Hugo PeluffoBerta González
Apr 24, 2002·Pulmonary Pharmacology & Therapeutics·J VadolasP A Ioannou
Jun 9, 2006·The Journal of General Virology·Li LiFeng Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.