A recombinant canine distemper virus expressing a modified rabies virus glycoprotein induces immune responses in mice

Virus Genes
Zhili LiWeiquan Liu

Abstract

Canine distemper virus (CDV) and rabies virus (RV) are two important pathogens of the dog. CDV, a member of the morbillivirus genus, has shown promise as an expression vector. The glycoprotein from RV is a main contributor to protective immunity and capable of eliciting the production of virus-neutralizing antibodies. In this study, we recovered an attenuated strain of canine distemper virus and constructed a recombinant virus, rCDV-RV-G, expressing a modified (R333Q) rabies virus glycoprotein (RV-G) of RV Flury strain LEP. RV-G expression by the recombinant viruses was confirmed. Furthermore, G was proved to be incorporated into the surface of CDV particles. While replication of the recombinant virus was slightly reduced compared with the parental CDV, it stably expressed the RV-G over ten serial passages. Inoculation of mice induced specific neutralizing antibodies against both RV-G and CDV. Therefore, the rCDV-RV-G has the potential as a vaccine that may be used to control rabies virus infection in dogs and other animals.

References

Nov 19, 1981·Nature·A AnilionisP J Curtis
Jan 1, 1995·Archives of Virology·J M Coll
Jun 1, 1995·Virology·Z Q XiangH C Ertl
May 1, 1995·Veterinary Microbiology·G Chappuis
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·T MebatsionK K Conzelmann
Feb 6, 1999·The Journal of General Virology·M Singh, M A Billeter
Nov 2, 2001·Journal of Virology·T NakayaP Palese
Sep 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Veronika von MesslingRoberto Cattaneo
Nov 21, 2008·Veterinary Immunology and Immunopathology·A BeinekeW Baumgärtner

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