A recombinant cell bioassay for measurement of overall estrogenic activity of serum: preliminary results in women with breast cancer

Clinical Chemistry and Laboratory Medicine : CCLM
Sophie Séronie-VivienJean-Charles Faye

Abstract

The estrogenic status of patients with breast cancer may influence the prognosis and the response to treatment and is currently assessed by immunological measurement of serum estradiol. This does not account for estrogenic or anti-estrogenic activity related to growth factors able to activate the estrogen receptor, to anti-estrogenic drugs or to exogenous supply of estrogen-like compounds. We developed a recombinant bioassay based on a mammary cell line expressing luciferase in an estrogen receptor-dependent way. In a human serum matrix the MELN system was able to detect the transcriptional activity of estradiol, growth factors (epidermal growth factor (EGF), insulin at insulin-like growth factor 1 (IGF-1)-like concentrations), xeno-estrogens (diethylstilbestrol, phytoestrogens) and tamoxifen in a dose-dependent manner. The intra- and inter-assay variations were < 6% and < or = 15%, respectively, whatever the estradiol concentration; the functional sensitivity was < 10 pmol/l equivalents of estradiol. We assessed the overall estrogenic activity of serum (OEAS) in 16 healthy women and in 24 women with advanced breast cancer. The correlation between OEAS and serum log10 17-beta-estradiol (E2) was good for healthy women (r2=0.8568...Continue Reading

References

Dec 1, 1994·The Journal of Clinical Investigation·K O KleinG B Cutler
Mar 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·M K El-Tanani, C D Green
May 7, 1999·Environmental Health Perspectives·H R AndersenA M Soto
May 20, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·J LeglerB van der Burg
Sep 24, 1999·The Science of the Total Environment·P BalaguerC Casellas
Mar 15, 2000·Molecular and Cellular Endocrinology·B TérouanneC Sultan
Nov 10, 2000·International Journal of Cancer. Journal International Du Cancer·P TonioloA Zeleniuch-Jacquotte
Feb 12, 2002·The Journal of Clinical Endocrinology and Metabolism·Françoise ParisCharles Sultan
Feb 8, 2003·Molecular and Cellular Endocrinology·Françoise ParisCharles Sultan
Mar 21, 2003·Journal of Proteome Research·Ronald L WoodburyRichard C Zangar

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Citations

Sep 16, 2020·The Science of the Total Environment·Marta GeaTiziana Schilirò

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Methods Mentioned

BETA
ELISA

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