A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV

Journal of Virology
Zhuhui HuangSiba K Samal

Abstract

Infectious bursal disease virus (IBDV) causes a highly immunosuppressive disease in chickens. Currently available, live IBDV vaccines can lead to generation of variant viruses. We have developed an alternative vaccine that will not create variant IBDV. By using the reverse genetics approach, we devised a recombinant Newcastle disease virus (NDV) vector from a commonly used vaccine strain LaSota to express the host-protective immunogen VP2 of a variant IBDV strain GLS-5. The gene encoding the VP2 protein of the IBDV was inserted into the most 3'-proximal locus of a full-length NDV cDNA for high-level expression. We successfully recovered the recombinant virus, rLaSota/VP2. The rLaSota/VP2 was genetically stable, at least up to 12 serial passages in chicken embryos, and was shown to express the VP2 protein. The VP2 protein was not incorporated into the virions of recombinant virus. Recombinant rLaSota/VP2 replicated to a titer similar to that of parental NDV strain LaSota in chicken embryos and cell cultures. To assess protective efficacy of the rLaSota/VP2, 2-day-old specific-pathogen-free chickens were vaccinated with the recombinant virus and challenged with a highly virulent NDV strain Texas GB or IBDV variant strain GLS-5 at...Continue Reading

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Citations

Aug 4, 2006·Veterinary Research Communications·V Balamurugan, J M Kataria
Aug 21, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Adam VigilAdolfo García-Sastre
Apr 21, 2012·Avian Pathology : Journal of the W.V.P.A·Hermann MüllerM Rafiqul Islam
Oct 17, 2006·Journal of Virology·Alexander BukreyevPeter L Collins
Jan 18, 2008·Journal of Virology·Qinshan GaoPeter Palese
Feb 25, 2011·PloS One·Sunil K KhattarSiba K Samal
Feb 14, 2013·Avian Diseases·Amélyne LardinoisThierry van den Berg
Mar 5, 2014·Virus Research·Ketan GanarSachin Kumar
Jul 17, 2012·Veterinary Immunology and Immunopathology·Satya Narayan PradhanUsha Antony

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