A recombinant protein based on the Trypanosoma cruzi metacyclic trypomastigote 82-kilodalton antigen that induces and effective immune response to acute infection.

Infection and Immunity
R Santori FN Yoshida

Abstract

To further investigate the immunological properties of the stage-specific 82-kDa glycoprotein (gp82) of Trypanosoma cruzi metacyclic trypomastigotes, previously shown to induce antigen-specific humoral and T-cell responses in mice, we performed a series of experiments with recombinant proteins containing sequences of gp82 fused to glutathione S-transferase. Of five fusion proteins tested, only J18b and J18b1, the carboxyproximal peptides containing amino acids 224 to 516 and 303 to 516, respectively, were recognized by monoclonal antibody 3F6 as well as by various anti-T. cruzi antisera and, when administered to mice, were capable of eliciting antibodies directed to the native gp82. The amino-terminal peptide and other carboxyterminal recombinant proteins lacking the central domain of gp82 (amino acids 224 to 356), which is exposed on the surface of live metacyclic forms, did not display any of these properties. Spleen cells derived from mice immunized with any of the five recombinant proteins proliferated in vitro in the presence of native gp82.J18b was the most stimulatory, whereas J18b3, the peptide containing amino acids 408 to 516, elicited the weakest response. When BALB/c mice immunized with J18b antigen plus A1(OH)3 as ...Continue Reading

References

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Citations

Aug 26, 2003·Revista Da Sociedade Brasileira De Medicina Tropical·Valéria Rêgo Alves PereiraYara de Miranda Gomes
Dec 23, 2003·Infection and Immunity·Eric DumonteilMaria Jesus Ramirez-Sierra
Jun 14, 2008·Clinical and Vaccine Immunology : CVI·Vandanajay Bhatia, Nisha Jain Garg
Mar 9, 2010·PLoS Neglected Tropical Diseases·Daniela I StaquiciniNobuko Yoshida
Sep 14, 2010·Memórias do Instituto Oswaldo Cruz·Christopher S EickhoffDaniel F Hoft
Dec 24, 2005·Expert Review of Vaccines·Nisha Garg, Vandanajay Bhatia
Aug 14, 2010·PLoS Neglected Tropical Diseases·Shivali Gupta, Nisha Jain Garg
May 25, 2007·FEMS Immunology and Medical Microbiology·Gilma Sanchez-BurgosEric Dumonteil
May 16, 2019·Scientific Reports·Esteban M CorderoJosé Franco da Silveira

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