A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge

Veterinary Immunology and Immunopathology
Zhi-Jun TianGuang-Zhi Tong

Abstract

The hemagglutinin (HA) gene of A/Swine/Inner Mogolian/547/2001 (H3N2) swine influenza virus (SIV) was recombined into the genome of pseudorabies virus (PRV) Bartha-K61 vaccine strain, generating a recombinant PRV expressing the HA gene, designated as rPRV-HA. One group of 15 mice was inoculated intranasally (i.n.) with 10(5.0) PFU of rPRV-HA, and another two control groups of mice (15 mice per group) were mock-inoculated or inoculated with Bartha-K61. Mice inoculated with rPRV-HA developed hemagglutination inhibition antibodies 3 weeks post-inoculation. Twenty-eight days post-inoculation, all mice were challenged i.n. with 10(5.0) TCID50 of A/Swine/Heilongjiang/74/2000 (H3N2). No challenge virus was isolated from vaccinated mice, and mild pathological lesions were observed only in lungs following challenge. The results demonstrate that the recombinant rPRV-HA expressing the HA gene from H3N2 SIV can protect mice from heterologous virulent challenge, and may represent a candidate vaccine against SIV.

References

Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·I J YoonG D Dial
Aug 23, 2000·Journal of Virology·R J WebbyR G Webster

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Citations

Jan 16, 2014·Indian Journal of Microbiology·Weili KongJuan Pu
May 14, 2010·Animal Health Research Reviews·Wenjun Ma, Jürgen A Richt
Dec 21, 2012·Animal Health Research Reviews·Qi ChenD L Hank Harris
Nov 18, 2008·Research in Veterinary Science·Guo-Xin LiGuang-Zhi Tong
Sep 6, 2008·Developmental and Comparative Immunology·Kenneth C McCullough, Artur Summerfield
Sep 25, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Thierry van den BergMichel Bublot
Jul 6, 2014·Journal of Immunology Research·Bo DongXiaofeng Ren
Jul 28, 2016·Human Vaccines & Immunotherapeutics·Rory D de Vries, Guus F Rimmelzwaan
May 14, 2021·BMC Veterinary Research·Chuanjian ZhangJichun Wang

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