A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague

Vaccines
Tonie E RockeJorge E Osorio

Abstract

In previous studies, we demonstrated in mice and prairie dogs that simultaneous administration of two recombinant raccoon poxviruses (rRCN) expressing Yersinia pestis antigens (F1 and V307-a truncated version of the V protein) provided superior protection against plague challenge compared to individual single antigen constructs. To reduce costs of vaccine production and facilitate implementation of a sylvatic plague vaccine (SPV) control program for prairie dogs, a dual antigen construct is more desirable. Here we report the construction and characterization of a novel RCN-vectored vaccine that simultaneously expresses both F1 and V307 antigens. This dual antigen vaccine provided similar levels of protection against plague in both mice and prairie dogs as compared to simultaneous administration of the two single antigen constructs and was also shown to protect mice against an F1 negative strain of Y. pestis. The equivalent safety, immunogenicity and efficacy profile of the dual RCN-F1/V307 construct warrants further evaluation in field efficacy studies in sylvatic plague endemic areas.

References

Feb 1, 2003·Vaccine·Jorge E OsorioDan T Stinchcomb
Oct 9, 2004·Annual Review of Entomology·Kenneth L Gage, Michael Y Kosoy
Jul 26, 2005·Infection and Immunity·Katie A OverheimOlaf Schneewind
Oct 30, 2008·Journal of Wildlife Diseases·Tonie E RockeJorge E Osorio
Dec 23, 2009·PLoS Neglected Tropical Diseases·Dennis SlateCharles E Rupprecht
Feb 18, 2010·Vector Borne and Zoonotic Diseases·Marc R MatchettTonie Rocke

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Citations

Aug 19, 2015·Parasitology·Hamish McCALLUM
Apr 1, 2017·Journal of Wildlife Diseases·Lucila M CorroMichael W Miller
Nov 22, 2017·EcoHealth·Rachel C AbbottTonie E Rocke
Feb 23, 2019·NPJ Vaccines·Wei Sun, Amit K Singh
Nov 7, 2019·Ecology and Evolution·Robin E RussellTonie E Rocke
Dec 27, 2016·Frontiers in Immunology·Shailendra Kumar Verma, Urmil Tuteja
May 28, 2019·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Jeffrey D Roth
Aug 25, 2021·ILAR Journal·Elizabeth A FalendyszJonathan M Sleeman

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Methods Mentioned

BETA
PCR

Software Mentioned

SAS

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