A recombinant Rickettsia conorii vaccine protects guinea pigs from experimental boutonneuse fever and Rocky Mountain spotted fever.

Infection and Immunity
S VishwanathN G Watkins

Abstract

There are no vaccines against boutonneuse fever and Rocky Mountain spotted fever. Previous studies have identified a Rickettsia rickettsii surface protein as a vaccine candidate and shown that an antigenically related protein is present in R. conorii, which causes boutonneuse fever. The gene encoding the R. rickettsii protein has been cloned and expressed in Escherichia coli. We confirmed by 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis of rickettsial lysates followed by immunoblotting with a monoclonal antibody raised against the R. rickettsii protein that an analogous protein exists in R. conorii. Although these proteins were previously called 155-kilodalton (kDa) proteins, we found that their apparent molecular masses were 198 kDa for R. conorii Kenya tick typhus and 190 kDa for R. rickettsii R. Using the R. rickettsii gene probe, we cloned and expressed a 5.5-kilobase HindIII fragment from R. conorii Kenya tick typhus genomic DNA in E. coli JM107. The expressed recombinant product was recognized by a monospecific polyclonal rabbit antiserum prepared against the 198-kDa protein. Guinea pigs immunized with sonic lysates of the E. coli strain expressing the recombinant gene product developed antibodies recogni...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1975·Journal of Clinical Microbiology·R H Kenyon, C E Pedersen
Jan 1, 1976·Journal of Clinical Microbiology·R N PhilipW Burgdorfer
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N H Carbonetti, P F Sparling
Dec 1, 1988·The American Journal of Medicine·J C McDonaldJ E McDade
Jun 1, 1985·The Journal of Infectious Diseases·R L AnackerL A Thomas
May 1, 1972·Infection and Immunity·D A WikeR A Ormsbee
Oct 19, 1967·The New England Journal of Medicine·J E Johnson, P J Kadull
Jan 1, 1983·Methods in Enzymology·J Messing
Mar 18, 1982·Nature·D Lane, H Koprowski

❮ Previous
Next ❯

Citations

Jun 24, 2003·Infection and Immunity·Zhen LiDavid H Walker
Oct 3, 2009·Infection and Immunity·Walairat PornwiroonKevin R Macaluso
Feb 17, 2006·Annals of the New York Academy of Sciences·Yanmei JiaoLing Qiu
Jun 23, 2009·Annals of the New York Academy of Sciences·Premanand BalrajDidier Raoult
Jan 1, 1993·Microbiology and Immunology·T Uchida
Jul 16, 2003·Annals of the New York Academy of Sciences·P Renesto, D Raoult
Jul 1, 2009·Microbiology and Immunology·Eun-Ju DoWon-Jong Jang
Feb 17, 2006·Annals of the New York Academy of Sciences·Tsuneo Uchiyama
Mar 30, 2011·Infection and Immunity·Yvonne Gar-Yun ChanJuan José Martinez
Jun 24, 2005·Molecular Biology and Evolution·Guillaume BlancDidier Raoult
Oct 23, 2020·PLoS Neglected Tropical Diseases·Anke Osterloh
Feb 1, 1992·Infection and Immunity·B ShankarappaB Mattingly-Napier
Dec 1, 1996·Infection and Immunity·M A KuzykW W Kay
Jun 11, 2021·PloS One·Jessica RauchAnke Osterloh
Apr 1, 2006·Research in Microbiology·Patricia RenestoDidier Raoult
Mar 1, 2006·BMC Microbiology·Maxime NgwamidibaPierre-Edouard Fournier
Aug 4, 2015·Ticks and Tick-borne Diseases·Walairat PornwiroonKevin R Macaluso

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.