A recombinant vaccine based on domain II of Plasmodium vivax Apical Membrane Antigen 1 induces high antibody titres in mice

Vaccine
Fernanda GentilIrene S Soares

Abstract

The Apical Membrane Antigen 1 (AMA-1) is considered a promising candidate for development of a malaria vaccine against asexual stages of Plasmodium. We recently identified domain II (DII) of Plasmodium vivax AMA-1 (PvAMA-1) as a highly immunogenic region recognised by IgG antibodies present in many individuals during patent infection with P. vivax. The present study was designed to evaluate the immunogenic properties of a bacterial recombinant protein containing PvAMA-1 DII. To accomplish this, the recombinant protein was administered to mice in the presence of each of the following six adjuvants: Complete/Incomplete Freund's Adjuvant (CFA/IFA), aluminium hydroxide (Alum), Quil A, QS21 saponin, CpG-ODN 1826 and TiterMax. We found that recombinant DII was highly immunogenic in BALB/c mice when administered in the presence of any of the tested adjuvants. Importantly, we show that DII-specific antibodies recognised the native AMA-1 protein expressed on the surface of P. vivax merozoites isolated from the blood of infected patients. These results demonstrate that a recombinant protein containing PvAMA-1 DII is immunogenic when administered in different adjuvant formulations, and indicate that this region of the AMA-1 protein should...Continue Reading

References

Nov 15, 1996·The Journal of Biological Chemistry·A N HodderR F Anders
Apr 9, 2001·Infection and Immunity·A N HodderR F Anders
Dec 17, 2003·The Journal of Biological Chemistry·Olivier SilvieDominique Mazier
Feb 16, 2005·International Journal for Parasitology·Maria Helena C RodriguesIrene S Soares
Feb 26, 2005·Science·Juan Carlos PizarroGraham A Bentley
Sep 13, 2005·Molecular and Biochemical Parasitology·Marie-Laure Chesne-SeckGraham A Bentley
May 6, 2006·Trends in Parasitology·Spencer D PolleyColin J Sutherland
Jan 30, 2007·Trends in Parasitology·Sócrates HerreraMyriam Arévalo-Herrera
Oct 16, 2007·Trends in Parasitology·J Kevin Baird
Nov 21, 2007·Trends in Parasitology·Chetan E Chitnis, Amit Sharma
Jan 30, 2008·Trends in Parasitology·Edmond J RemarqueAlan W Thomas
Jun 28, 2008·The Lancet Infectious Diseases·Lionel K K TanMichael Jacobs
Sep 2, 2008·Trends in Parasitology·Brian M Greenwood
Feb 20, 2009·Malaria Journal·Mary R Galinski, John W Barnwell
Jan 24, 2009·PLoS Pathogens·Christine R CollinsMichael J Blackman
Apr 8, 2009·Trends in Parasitology·Nicholas M AnsteyRic N Price
Aug 1, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Chaturong PutaporntipAustin L Hughes

❮ Previous
Next ❯

Citations

Mar 5, 2013·PloS One·Amanda R BitencourtIrene S Soares
Nov 28, 2012·Malaria Journal·Ana Cecilia LopezGustavo A Fontecha
May 22, 2013·Malaria Journal·Darwin A Moreno-PérezManuel A Patarroyo
Feb 7, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Germain J P FernandoMark A F Kendall
Aug 15, 2014·Frontiers in Immunology·Alyssa E Barry, Alicia Arnott
Nov 28, 2012·Expert Review of Vaccines·Manuel A PatarroyoDarwin A Moreno-Pérez
Mar 1, 2017·Frontiers in Immunology·Carolina LópezManuel A Patarroyo
Jan 23, 2021·Expert Review of Vaccines·Sai Lata DeJohn H Adams

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.