A red-emissive antibody-AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells

Chemical Science
Xiujuan ShiBen Zhong Tang

Abstract

An antibody-AIEgen conjugate is designed and developed as a "turn-on" fluorescent probe for wash-free specific cancer cell imaging. The cetuximab-conjugated AIEgen shows red fluorescence only when it is internalized and accumulated in cancer cells with overexpressed epidermal growth factor receptor through endocytosis. The probe first lights up the lysosomes. After hydrolysis, its residue is accumulated in mitochondria, making them highly emissive with a long cell retention time. Compared with conventional "always-on" fluorescent probes, the antibody-AIEgen conjugate exhibits a very good image contrast during wash-free cancer cell imaging and less interference from normal cells. To the best of our knowledge, this is the first time "turn-on" antibody-AIEgen conjugates have been reported. This new strategy can be further extended to many proteins and water-soluble AIEgens, and many of their potential applications such as real-time tracking of cell dynamics and cancer theranostics will be explored. The present work is expected to inspire more marvellous research in the fields of AIE and cancer imaging.

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Citations

Jan 3, 2019·Chemistry : a European Journal·Sheng XieBen Zhong Tang
Jul 22, 2018·Advanced Science·Zhen WangChun Zhang
Sep 28, 2018·Advanced Healthcare Materials·Xiaowen LiuZhuang Liu
Oct 13, 2018·Chemical Science·Yuan GuBen Zhong Tang
Feb 16, 2021·Journal of Drug Targeting·Rashmi Kumari, Dhanya Sunil
Mar 18, 2021·Colloids and Surfaces. B, Biointerfaces·Wenli FengXing Wang
May 25, 2021·Chemical Society Reviews·Hao-Ran JiaFu-Gen Wu
Feb 11, 2020·Chemical Science·Xiujuan ShiBen Zhong Tang
Nov 16, 2017·ACS Applied Materials & Interfaces·Ju MeiHe Tian
Aug 23, 2019·Accounts of Chemical Research·Dong Wang, Ben Zhong Tang

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Methods Mentioned

BETA
X-ray
imaging techniques
fluorescence resonance
FRET
NMR
fluorescence imaging
fluorescence
confocal microscopy
flow cytometry
electrophoresis

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