A Red/Green Cyanobacteriochrome Sustains Its Color Despite a Change in the Bilin Chromophore's Protonation State

Biochemistry
Chen SongPeter Hildebrandt

Abstract

The second GAF domain of AnPixJ, AnPixJg2, a bilin-binding protein from the cyanobacterium Anabaena PCC 7120, undergoes a photoinduced interconversion between a red-absorbing state, Pr, and a green-absorbing state, Pg. Combining ultraviolet-vis (UV-vis), infrared, resonance Raman (RR), and magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy, we have studied this cyanobacteriochrome (CBCR) assembled with phycocyanobilin (PCB) either in vivo or in vitro. In both assembly routes, the spectroscopic data of the Pr state reveal nearly identical chromophore structures with a protonated (cationic) bilin. However, unlike the native (in vivo assembly) Pg photoproduct, in which the bilin retains protonation, the Pg generated from the in vitro-assembled AnPixJg2 harbors a deprotonated (neutral) bilin chromophore at pH 7.8. IR difference spectroscopy further reveals the transfer of a proton from the bilin to a side-chain carboxylate on an amino acid, probably Asp291. Besides the change in protonation state, the bilin structure is very similar in the in vitro- and in vivo-assembled Pg photoproducts. The chromophore of the in vitro Pg becomes protonated when the pH is increased to 10, presumably because of a partial rever...Continue Reading

References

Mar 14, 2002·Chemical Reviews·Waclaw Kolodziejski, Jacek Klinowski
Aug 3, 2005·The Journal of Biological Chemistry·Berthold BoruckiPeter Hildebrandt
Jul 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Anna DillerA Alia
Jun 24, 2008·Journal of Molecular Biology·Rei NarikawaMasahiko Ikeuchi
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lars-Oliver EssenJon Hughes
Oct 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Thierry RohmerJörg Matysik
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Masahiko Ikeuchi, Takami Ishizuka
Feb 16, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Nathan C Rockwell, J Clark Lagarias
Feb 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chen SongJörg Matysik
Nov 16, 2012·Biochemistry·Nathan C RockwellJ Clark Lagarias
Dec 12, 2012·Photochemistry and Photobiology·Chen SongJörg Matysik
Dec 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rei NarikawaMasahiko Ikeuchi
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yuu HiroseMasahiko Ikeuchi
Apr 23, 2013·The Journal of Biological Chemistry·Johannes SalewskiPeter Hildebrandt
Dec 18, 2013·The Journal of Biological Chemistry·Claudia C CornilescuRichard D Vierstra
Dec 25, 2013·Chemical Reviews·Oliver P ErnstHideki Kandori
Apr 30, 2014·Nature·Heikki TakalaSebastian Westenhoff
Oct 25, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Nathan C RockwellJ Clark Lagarias
Apr 23, 2015·Nature Chemistry·Francisco Velazquez EscobarPeter Hildebrandt

❮ Previous
Next ❯

Citations

Dec 15, 2016·The Journal of Physical Chemistry. B·Francisco Velazquez EscobarMaria Andrea Mroginski
May 14, 2017·Photochemistry and Photobiology·Keiji FushimiRei Narikawa
Jan 6, 2017·Photochemistry and Photobiology·Nathan C RockwellJohn Clark Lagarias
May 18, 2017·Physical Chemistry Chemical Physics : PCCP·Laura Katharina Scarbath-EversDaniel Sebastiani
Aug 5, 2017·International Journal of Molecular Sciences·Olena S OliinykVladislav V Verkhusha
Apr 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Sunghyuk LimJames B Ames
Jul 31, 2019·International Journal of Molecular Sciences·Qian-Zhao XuChen Song
May 14, 2017·Photochemistry and Photobiology·Tilman LamparterPatrick Scheerer
Oct 28, 2019·The Journal of Biological Chemistry·Teppei SatoYuu Hirose
Sep 3, 2020·International Journal of Molecular Sciences·Keiji FushimiRei Narikawa
Dec 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sascha Jähnigen, Daniel Sebastiani
Jan 1, 2021·The Journal of Chemical Physics·David BuhrkePeter Hamm
Apr 27, 2021·Physical Chemistry Chemical Physics : PCCP·Jeannette RufDavid Buhrke
Apr 21, 2021·Physical Chemistry Chemical Physics : PCCP·Aditya G RaoIgor Schapiro
May 12, 2021·Proceedings of the National Academy of Sciences of the United States of America·Takayuki NagaeMasaki Mishima

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.