PMID: 6103519Mar 1, 1980Paper

A reevaluation of veratridine as a tool for studying the depolarization-induced release of neurotransmitters from nerve endings

Neurochemical Research
G LeviM Raiteri

Abstract

The effect of veratridine on neurotransmitter release was studied using rat brain synaptosomes superfused at 37 degrees C. Veratridine (5-75 microM) caused a concentration-dependent release of [3H]GABA from prelabeled synaptosomes in the presence of 2.7 mM Ca2+. In the whole range of veratridine concentrations, the release of [3H]GABA elicited by the drug was substantially increased rather than decreased in the absence of Ca2+ or with Ca2+ concentrations of 0.45 and 0.9 mM. The release of the amino acid was inhibited more by 5.4 mM than by 2.7 mM Ca2+. The effect on endogenous (chemically measured) GABA was similar to that on [3H]GABA. The inhibitory effect of Ca2+ on the veratridine-induced release of [3H]GABA was consistently seen in a variety of experimental conditions except one, namely when the experiment was run at room temperature (22-23 degrees C) rather than at physiological temperature (37 degrees C). In fact, at 22-23 degrees C the release of GABA evoked by the alkaloid was somewhat potentiated by Ca2+. At 37 degrees C, glutamate appeared to behave similarly to GABA, whereas the veratridine-induced release of [3H]noradrenaline and [3H]dopamaine was largely Ca2+-dependent. The mechanism of the release of transmitters ...Continue Reading

References

Aug 1, 1977·Journal of Neurochemistry·J S De Belleroche, H F Bradford
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G Levi, M Raiteri
Nov 1, 1979·Journal of Neurochemistry·G LeviM Raiteri
Jan 1, 1979·The Journal of Physiology·W B Stallcup
Jul 9, 1976·Brain Research·G A Goddard, J D Robinson
Jun 1, 1975·Journal of Neurochemistry·M RaiteriG Levi
May 1, 1975·Biochemical Pharmacology·L L Iversen, J S Kelly
Jan 16, 1976·Brain Research·F A HennD G Rustad
Mar 1, 1974·European Journal of Pharmacology·M RaiteriG Levi
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M P BlausteinP Needleman

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Citations

Mar 31, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·M KammererThomas J Feuerstein
May 3, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·M KammererT J Feuerstein
Nov 4, 1986·European Journal of Pharmacology·E J White, H F Bradford
Sep 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·J V MartinW O McClure
Jan 1, 1992·Progress in Neurobiology·P Saransaari, S S Oja
Jun 16, 2000·European Journal of Pharmacology·K OkuyamaY Kudo
Dec 1, 1999·British Journal of Pharmacology·F A Jones, J A Davies
Sep 1, 1983·Clinical and Experimental Pharmacology & Physiology·J H Skerritt, G A Johnston
Feb 1, 1991·Acta Physiologica Scandinavica·M Pérez de la MoraK Fuxe
Feb 1, 1992·Journal of Neurochemistry·V Adam-Vizi
Jul 11, 2014·Journal of Neural Transmission·Katharina PraussAndreas Moser
Oct 1, 1989·Journal of Neurochemistry·G Levi, M Raiteri
Jul 1, 1987·Journal of Neurochemistry·J Sanchez-PrietoD G Nicholls
Jan 30, 2016·Journal of Neurochemistry·Michael P RassnerThomas J Feuerstein
Jul 1, 1996·Vision Research·C B DuarteA P Carvalho

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