A region of human CD14 required for lipopolysaccharide binding.

The Journal of Biological Chemistry
S Viriyakosol, T N Kirkland

Abstract

CD14, a glycosylphosphatidylinositol-anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, is a receptor for lipopolysaccharide (LPS). CD14 binding of LPS is enhanced by serum proteins, especially lipopolysaccharide binding protein. The serum-dependent binding of LPS to CD14 stimulates macrophages to make cytokines, which can cause septic shock in humans and animals. Here, we identify a region in human CD14 which is important in serum-dependent LPS binding and LPS-induced cellular activation. Four small regions (4-5 amino acids long) within the N-terminal 65 amino acids of CD14 were deleted singly or in combination. The deletion mutants were stably expressed in Chinese hamster ovary (CHO) cells. The mutants were characterized in three assays: reactivity with anti-CD14 monoclonal antibody, serum-dependent LPS binding, and LPS-induced activation of NF-kappa B. Some of the mutants selectively lost reactivity with the anti-CD14 monoclonal antibody that inhibited serum-dependent LPS binding and cellular activation. All of the mutants bound much less LPS than wild type CD14 in the presence of serum. None of the mutants bound more LPS than control CD14-CHO cells in the absence of serum. CD14-CHO ...Continue Reading

References

Mar 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G WakabayashiC A Dinarello
Feb 12, 1987·The New England Journal of Medicine·B Beutler, A Cerami
Jan 1, 1994·The Journal of Experimental Medicine·E HailmanS D Wright
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J PuginP S Tobias
Jan 1, 1993·Advances in Immunology·R J Ulevitch
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J D LeeR J Ulevitch

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Citations

Dec 1, 1995·Archives of Microbiology·J SchletterE T Rietschel
Jan 1, 1996·Progress in Biophysics and Molecular Biology·S G Buchanan, N J Gay
May 26, 1999·Infectious Disease Clinics of North America·R R IngallsD Golenbock
Mar 6, 1999·Current Opinion in Microbiology·R P Darveau
Mar 29, 2001·Nature Immunology·K TriantafilouR L Dedrick
Jan 1, 1997·Journal of Periodontal Research·P S TobiasR J Ulevitch
Oct 1, 1996·The Journal of Clinical Investigation·D J LeturcqR J Ulevitch
Dec 2, 2011·Molecular Biology International·Aruna PalA K Chakravarty
Dec 16, 2006·Developmental and Comparative Immunology·Gema SanzJuan J Garrido
Jan 31, 2006·Chembiochem : a European Journal of Chemical Biology·Söhnke VossRoland Brock
Apr 21, 2016·Journal of Lipid Research·Pitchaimani KandasamyDennis R Voelker
May 16, 1998·The Journal of Biological Chemistry·R DziarskiP S Tobias
Dec 1, 2001·Nature Genetics·V L Waters
Feb 10, 2006·The Journal of Biological Chemistry·Suganya ViriyakosolTheo N Kirkland
Aug 28, 2002·The Journal of Biological Chemistry·Masashi MuroiKen-Ichi Tanamoto
Sep 20, 1996·The Journal of Biological Chemistry·D GuptaR Dziarski
May 13, 1999·The Journal of Biological Chemistry·D GuptaR Dziarski
Feb 1, 2000·The Journal of Biological Chemistry·S ViriyakosolT N Kirkland
Nov 14, 2001·The Journal of Biological Chemistry·Jean da Silva Correia, Richard J Ulevitch
Dec 25, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stacy L KelleyRichard I Tapping

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