A regulatory circuit of two lncRNAs and a master regulator directs cell fate in yeast

Nature Communications
Fabien MorettoFolkert J van Werven

Abstract

Transcription of long noncoding RNAs (lncRNAs) regulates local gene expression in eukaryotes. Many examples of how a single lncRNA controls the expression of an adjacent or nearby protein-coding gene have been described. Here we examine the regulation of a locus consisting of two contiguous lncRNAs and the master regulator for entry into yeast meiosis, IME1. We find that the cluster of two lncRNAs together with several transcription factors form a regulatory circuit by which IME1 controls its own promoter and thereby promotes its own expression. Inhibition or stimulation of this unusual feedback circuit affects timing and rate of IME1 accumulation, and hence the ability for cells to enter meiosis. Our data demonstrate that orchestrated transcription through two contiguous lncRNAs promotes local gene expression and determines a critical cell fate decision.

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Citations

Nov 6, 2018·Nature Communications·Peter KindgrenSebastian Marquardt
Sep 3, 2020·Journal of Fungi·Jürgen Wendland
Jan 20, 2020·Functional & Integrative Genomics·Mohamad Al KadiDaisuke Okuzaki
Jun 8, 2019·Chemical Communications : Chem Comm·Linqiang PanCheng Zhang
Jul 28, 2020·Frontiers in Bioengineering and Biotechnology·Yongcan ChenTong Si
May 10, 2020·Nature Communications·Janis Tam, Folkert J van Werven
Oct 9, 2020·Genes & Genetic Systems·Satoshi Senmatsu, Kouji Hirota
Feb 19, 2021·Biological Reviews of the Cambridge Philosophical Society·Igor StuparevićMichael Primig
May 11, 2021·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Marjolein E CrooijmansG Paul H van Heusden
Jul 3, 2021·Non-coding RNA·Vedrana Andric, Mathieu Rougemaille

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Methods Mentioned

BETA
PCR
immunoprecipitation
ChIP
smRNAFISH

Software Mentioned

element
MATLAB
ImageJ
NIS
StarSearch

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