Abstract
Protein expression changes in relation to cell cycles provide important information, and it may represent a new method for an early diagnosis of metaplasia - dysplasia - adenocarcinoma sequence. We investigated potential changes in cell cycle genes such as protooncogenes (PCNA, EGFR), tumour suppressor gene (p53), apoptotic TUNNEL (Tdt mediated dUTP nick and labelling) gene, as well as small intestinal mucus antigen (SIMA) and large intestinal mucus antigen (LIMA), which accumulates in metaplastic epithelium due to the inflammatory process in routine oesophageal biopsies using immunohistochemistry. Oesophageal biopsies were taken from patients with Barrett's oesophagus (n = 30), reflux oesophagitis (n = 30), healthy oesophagus (n = 30) and healthy cardia (n = 10). Immunohistochemical signalling was carried out by Streptavidin-Biotin-AEC (aminoetil-carbazol). Expression of PCNA was statistically significantly lower in healthy oesophagus (p < 0.05) versus reflux oesophagitis and Barrett's oesophagus. However, no significant change was detected in the expression of SIMA and LIMA in intestinal metaplasia. Further, EGFR, p53 and TUNNEL levels were significantly different in healthy versus Barrett's oesophagus. Manual counting using ...Continue Reading
References
Mar 18, 1999·The New England Journal of Medicine·J LagergrenO Nyrén
Feb 1, 2000·Journal of Cellular Physiology·T Scholzen, J Gerdes
Nov 15, 2000·Gut·G S ArulA P Corfield
Jul 27, 2001·Alimentary Pharmacology & Therapeutics·R F SouzaS J Spechler
Mar 15, 2002·The New England Journal of Medicine·Stuart Jon Spechler
Apr 10, 2002·American Journal of Clinical Pathology·Taofeek OwonikokoMario Sarbia
Jun 26, 2002·The British Journal of Surgery·G J S JenkinsJ N Baxter
Nov 20, 2002·European Journal of Gastroenterology & Hepatology·Jeanette CroftJames M Parry
Aug 15, 2003·World Journal of Surgery·Stig Ramel
Jun 1, 2004·The Journal of Biological Chemistry·Jennifer J RahnJudith C Hugh
Feb 16, 2005·Gut·J Lagergren
Feb 16, 2005·Gut·P Sharma, E I Sidorenko
Feb 16, 2005·Gut·J-F Fléjou
Mar 1, 2007·PLoS Medicine·Patricia C GalipeauBrian J Reid
Apr 3, 2007·Indian Journal of Cancer·S C BurjonrappaB Loggie
Jan 17, 2008·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·G SarosiStuart Jon Spechler
Dec 4, 2010·World Journal of Gastroenterology : WJG·Chin-Ann J OngRebecca C Fitzgerald
Apr 5, 2011·Journal of Surgical Oncology·Akinori EgashiraYoshihiko Maehara
May 25, 2011·Journal of Clinical Pathology·L H Moyes, J J Going
Sep 13, 2012·Endoscopy·H NeuhausB Schumacher