PMID: 7025903Apr 6, 1981Paper

A relationship between anion transport and a structural transition of the human erythrocyte membrane

Biochimica Et Biophysica Acta
J SnowJ F Brandts

Abstract

Scanning microcalorimetry was employed as an aid in examining some structural features of the anion transport system in red blood cell vesicles. Two structural transitions were previously shown to be sensitive to several covalent and non-covalent inhibitors of anion transport in red cells. In this study, these transitions were selectively removed, either thermally or enzymatically, and the subsequent effect on 35SO2- 4 efflux in red cell vesicles was determined. It is shown that removal of one of these transitions (B2) has a negligible inhibitory effect on anion transport. Cytoplasmic, intermolecular disulfide linkages between band 3 dimers are known to form during the B2 transition. The integrity of the 4,4-diisothiocyanostilbene-2,2-disulfonate-sensitive C transition, on the other hand, is shown to be a requirement for anion transport. The localized region of the membrane giving rise to this transition contains the transmembrane segment of band 3, as well as membrane phospholipids. The calorimetric results suggest a structure of band 3 which involves independent structural domains, and are consistent with the transmembrane segment playing a direct role in the transport process.

References

Jun 28, 1977·Biochemistry·E Strapazon, T L Steck
Jan 1, 1978·Journal of Supramolecular Structure·T L Steck
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M Kasahara, P C Hinkle
May 5, 1975·Biochemical and Biophysical Research Communications·A RothsteinR Juliano
Sep 11, 1973·Biochemistry·W M JacksonJ F Brandts
Jan 10, 1973·Journal of Molecular Biology·A IkaiC Tanford
Jan 1, 1973·Journal of Supramolecular Structure·T L Steck, J Yu
Jan 1, 1980·Annals of the New York Academy of Sciences·A Rothstein, M Ramjeesingh
Jan 1, 1963·Archives of Biochemistry and Biophysics·J T DODGED J HANAHAN

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Citations

Feb 1, 1997·Biulleten' eksperimental'noĭ biologii i meditsiny·A V MatveevV L Shnyrov
Nov 1, 1991·The International Journal of Neuroscience·A CupelloG Rialdi
Jul 17, 1995·Biochimica Et Biophysica Acta·T Haltia, E Freire
Feb 14, 1995·Biochimica Et Biophysica Acta·T Haltia, E Freire

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