A relative quantitative positive/negative ion switching method for untargeted lipidomics via high resolution LC-MS/MS from any biological source

Metabolomics : Official Journal of the Metabolomic Society
Susanne B BreitkopfJohn M Asara

Abstract

Advances in high-resolution mass spectrometry have created renewed interest for studying global lipid biochemistry in disease and biological systems. Here, we present an untargeted 30 min. LC-MS/MS platform that utilizes positive/negative polarity switching to perform unbiased data dependent acquisitions (DDA) via higher energy collisional dissociation (HCD) fragmentation to profile more than 1000-1500 lipid ions mainly from methyl-tert-butyl ether (MTBE) or chloroform:methanol extractions. The platform uses C18 reversed-phase chromatography coupled to a hybrid QExactive Plus/HF Orbitrap mass spectrometer and the entire procedure takes ~10 h from lipid extraction to identification/quantification for a data set containing 12 samples (~4 h for a single sample). Lipids are identified by both accurate precursor ion mass and fragmentation features and quantified using Lipid-Search and Elements software. Using this approach, we are able to profile intact lipid ions from up to 18 different main lipid classes and 66 subclasses. We show several studies from different biological sources, including cultured cancer cells, resected tissues from mice such as lung and breast tumors and biological fluids such as plasma and urine. Using mouse e...Continue Reading

References

Jun 23, 2007·Nucleic Acids Research·Eoin FahyShankar Subramaniam
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
May 31, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·B L KasiskeA Wilkinson
Sep 3, 2008·Cell Metabolism·Thomas PorstmannAlmut Schulze
Oct 28, 2008·Nucleic Acids Research·David S WishartIan Forsythe
Apr 30, 2009·Nucleic Acids Research·Chih-Hung ChouHsien-Da Huang
May 12, 2009·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Jun 17, 2010·Rapid Communications in Mass Spectrometry : RCM·Eva-Maria HeinHeiko Hayen
Jul 31, 2010·Molecular Cell·Katrin DüvelBrendan D Manning
Oct 19, 2010·Analytical and Bioanalytical Chemistry·Hye Kyeong MinMyeong Hee Moon
Dec 16, 2010·Rapid Communications in Mass Spectrometry : RCM·Weimin HouDaniel Figeys
Dec 21, 2010·Bioinformatics·Jürgen HartlerGerhard G Thallinger
May 10, 2011·Annual Review of Biochemistry·Jürgen Cox, Matthias Mann
May 26, 2011·Cold Spring Harbor Perspectives in Biology·Dominik SchwudkeAndrej Shevchenko
Oct 18, 2011·Expert Review of Proteomics·Kevin M Coombs
Dec 14, 2011·Analytica Chimica Acta·Georgios A TheodoridisIan D Wilson
Feb 22, 2012·Trends in Analytical Chemistry : TRAC·Jun Feng XiaoHabtom W Ressom
Nov 17, 2012·Journal of Proteomics·Stephen TateAnne-Claude Gingras
Jan 17, 2013·Genes & Development·Brandon N NicolayNicholas J Dyson
Feb 13, 2013·EMBO Reports·Stéphane J H Ricoult, Brendan D Manning
Aug 24, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Sin Man Lam, Guanghou Shui
Oct 22, 2013·Analytica Chimica Acta·Frans M van der KloetTheo Reijmers
Nov 13, 2013·Analytical Chemistry·Min LiHuwei Liu
Nov 30, 2013·Mass Spectrometry Reviews·Christophe JunotFrançois Bécher
Dec 18, 2013·Chemistry and Physics of Lipids·Yulia Y TyurinaValerian E Kagan
Jan 1, 2012·Metabolites·Harald C KöfelerMartin Trötzmüller
Aug 21, 2014·The New England Journal of Medicine·David J Kwiatkowski, Brendan D Manning
Oct 14, 2014·Trends in Analytical Chemistry : TRAC·Tomas Cajka, Oliver Fiehn
Nov 21, 2014·Journal of Inherited Metabolic Disease·Frédéric M VazAntoine H C van Kampen
Dec 3, 2014·Gene·Ana Emília Holanda RolimLuzimar Gonzaga Fernandez

❮ Previous
Next ❯

Citations

Mar 22, 2018·Bioanalysis·Jose Rodríguez-MoratóJosep Marcos
Jan 18, 2018·Nature Genetics·Ming ChenPier Paolo Pandolfi
Mar 23, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nacima AidoudJean-Charles Martin
Jan 15, 2020·Nature Chemical Biology·Timothy B WareKu-Lung Hsu
Feb 28, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Magali BarchukBénédicte Gaborit
Oct 14, 2017·PloS One·Min YuanJohn M Asara
Nov 8, 2017·Scientific Reports·Susanne B BreitkopfJohn M Asara
Feb 29, 2020·Metabolomics : Official Journal of the Metabolomic Society·P PousinisD A Barrett
Dec 12, 2018·Scientific Reports·Kévin ContrepoisMichael Snyder
Dec 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Giorgia ZadraMassimo Loda
Apr 2, 2020·Mass Spectrometry Reviews·Thomas Züllig, Harald C Köfeler
Feb 6, 2020·Nature Microbiology·Benedikt GeierManuel Liebeke
Aug 2, 2020·Rapid Communications in Mass Spectrometry : RCM·Mostafa J KhanRenã A S Robinson
Oct 21, 2020·Nucleic Acids Research·Xianping ShiH Phillip Koeffler
Nov 10, 2020·Science China. Life Sciences·Xiaohong ZhengGuangcun He
Jan 8, 2021·Experimental & Molecular Medicine·Julia LeskoKatharina Leithner
Mar 7, 2021·Toxins·Stephanie Mouchbahani-Constance, Reza Sharif-Naeini
May 6, 2020·Journal of Agricultural and Food Chemistry·Zhiqian LiuSimone Rochfort
Jun 18, 2020·ACS Omega·Zhiqian LiuSimone Rochfort
Mar 17, 2021·Nature Chemical Biology·Christopher F BennettPere Puigserver
Apr 6, 2021·Frontiers in Synaptic Neuroscience·Maria IulianoKimberly B Kegel-Gleason
May 1, 2021·Microorganisms·Sneider Alexander Gutierrez GuarnizoZemfira N Karamysheva
May 14, 2021·CA: a Cancer Journal for Clinicians·Daniel R SchmidtJason W Locasale
Apr 19, 2021·The Plant Journal : for Cell and Molecular Biology·Cheka KehelpannalaUte Roessner
Aug 18, 2021·Cancer & Metabolism·Daniel F LiefwalkerRosalie C Sears
Jan 28, 2020·Journal of Proteome Research·Kendra J AdamsUNKNOWN Alzheimer’s Disease Metabolomics Consortium
Aug 29, 2021·Chemistry and Physics of Lipids·Niels A W de KokArnold J M Driessen
Oct 27, 2021·Molecular Omics·Cheka KehelpannalaUte Roessner

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA

Software Mentioned

MetaboAnalyst
Elements for Metabolomics from Proteome Software
ProteomeSoftware
Excel
dChip
Proteome
R
Elements
Multi
XCalibur

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.