A reliable and simplified sj/beta-TREC ratio quantification method for human thymic output measurement

Journal of Immunological Methods
Sara Ferrando-MartínezManuel Leal

Abstract

Current techniques to peripherally assess thymic function are: the signal-joint T-cell receptor excision circle (sj-TREC) level measurement and the naive T cell and CD31+ TREC-rich subset determination. However, all of them are indirect approaches and none could be considered a direct recent thymic emigrant (RTE) marker. To overcome their limitations, Dion et al. (2004) described the sj/beta-TREC ratio that allows the peripheral quantification of the double negative to double positive intrathymic proliferation step. Nevertheless, the protocol described is expensive, sample and time-consuming, thus, limiting its usefulness. In this study, we describe a simplified protocol that reduces from 33 to 9 the amount of PCR reaction needed but maintaining the sensitivity and reproducibility of the original technique. In addition, we corroborated the effectiveness of our technique as an accurate thymic output-related marker by correlating the peripheral sj/beta-TREC ratio with a direct measurement of thymic function as the percentage of double positive thymocytes (r=0.601, p<0.001).

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Citations

Aug 12, 2010·Age·Sara Ferrando-MartínezManuel Leal
Feb 4, 2012·AIDS·Yue YangMathias Lichterfeld
Oct 12, 2012·Clinical and Vaccine Immunology : CVI·Sara CantisánJulián Torre-Cisneros
Aug 11, 2012·PloS One·Xue-ling OuHong-yu Sun
Nov 6, 2012·Annual Review of Immunology·Pamela J Fink
Nov 2, 2012·Immunity & Ageing : I & a·Rafael SolanaGraham Pawelec
Mar 5, 2013·Revista española de geriatría y gerontología·Sara Ferrando-MartínezM Ángeles Muñoz-Fernández
Sep 23, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sara Ferrando-MartínezManuel Leal
Mar 30, 2016·Biogerontology·James E Turner
Feb 16, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I Gracia-AhufingerJ Torre-Cisneros
Nov 6, 2012·Human Vaccines & Immunotherapeutics·Andres H GutiérrezAnnie S De Groot
Apr 14, 2016·The Journal of Infectious Diseases·Viviana Cobos JiménezUNKNOWN AGEhIV Study Group
Jun 24, 2016·Biodemography and Social Biology·Lydia FeinsteinAllison E Aiello
Dec 18, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·I Rosado-SánchezYolanda M Pacheco
Jul 18, 2019·Immunity & Ageing : I & a·I Herrero-FernándezY M Pacheco
Feb 6, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sara Ferrando-MartinezEzequiel Ruiz-Mateos
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May 14, 2020·Journal of Exposure Science & Environmental Epidemiology·Evans K LodgeAllison E Aiello
Jun 3, 2021·Cancers·Meriem Ben KhoudCarine Brinster
May 27, 2021·Aging·Vanesa Garrido-RodríguezYolanda María Pacheco

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