Abstract
In birds, offspring sex ratio manipulation by mothers is now well established with potentially important consequences for evolution and animal breeding. In most studies on primary sex ratio of birds, eggs are sexed after incubation by the use of PCR methods targeted to the sex-linked CHD1 genes. Sexing of unincubated eggs would be preferred, but as fertile and infertile blastodiscs cannot be distinguished macroscopically, errors could arise from PCR amplifications of parental DNA associated with the vitelline membrane of infertile eggs. In this study, we stained blastodiscs without the vitelline membrane with Hoechst 33342. This allowed unequivocal distinction between fertile and infertile blastodiscs. Fertile blastodiscs contained thousands of fluorescent nuclei, whereas no nuclei were seen in infertile eggs. In addition, after nucleic acid analysis, fertile blastodiscs yielded much stronger chromosomal DNA and CHD1-targeted PCR bands on agarose gels compared with infertile blastodiscs. These findings indicate that fertile blastodiscs contain much more embryonic DNA than parental DNA, allowing reliable sexing of the fertile eggs. The differences between fertile and infertile blastodiscs in chromosomal DNA and CHD1 PCR banding ...Continue Reading
References
Oct 1, 1992·Molecular Ecology·R GriffithsS A Becher
Jul 1, 1987·Journal of Reproduction and Fertility·G J Wishart
Jan 5, 1973·Science·R L Trivers, D E Willard
Jul 1, 1994·Journal of Reproduction and Fertility·T R BirkheadF Fletcher
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·H EllegrenB C Sheldon
Dec 16, 1998·Animal Behaviour·R Kilner
Aug 26, 1998·Molecular Ecology·R GriffithsR J Dawson
Mar 10, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S C ChapmanA Lumsden
Aug 3, 2001·Nature·M PetrieT Burke
Sep 14, 2001·Molecular Ecology·D S RichardsonT Burke
Nov 1, 2002·The Journal of Experimental Zoology·Pamela K Elf, Albert J Fivizzani
Nov 13, 2002·Proceedings. Biological Sciences·Wendt MüllerTon G G Groothuis
Aug 1, 1964·Journal of Reproduction and Fertility·L W BOBRF W LORENZ
Nov 25, 2003·Molecular Ecology·Kathryn E ArnoldRichard Griffiths
Dec 12, 2003·Proceedings. Biological Sciences·Kathryn E ArnoldDavid C Houston
Jan 1, 2004·Biological Reviews of the Cambridge Philosophical Society·Thomas W Pike, Marion Petrie
Mar 12, 2004·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A BorstA C Fluit
May 8, 2004·Journal of Clinical Microbiology·J HoorfarP Fach
Feb 14, 2006·The American Naturalist·Oliver P LoveTony D Williams
Apr 8, 2006·Proceedings. Biological Sciences·Thomas W Pike, Marion Petrie
Dec 7, 2006·Biology Letters·Thomas W Pike, Marion Petrie
Dec 7, 2006·Biology Letters·Stephanie M CorreaPatricia A Johnson
Dec 7, 2006·Biology Letters·Kevin M PilzHubert Schwabl
Oct 22, 2008·Poultry Science·W M LiS J Zhang
Apr 7, 2009·General and Comparative Endocrinology·V C GoerlichT G G Groothuis
Citations
Mar 21, 2013·General and Comparative Endocrinology·M Aamir AslamHenri Woelders
Oct 31, 2012·Molecular Ecology·Pierre De Wit, Stephen R Palumbi
May 29, 2016·Molecular Ecology Resources·A Centeno-CuadrosR Nathan
May 15, 2015·Biology of Reproduction·Muhammad Aamir AslamHenri Woelders
Feb 21, 2014·Biology of Reproduction·Muhammad Aamir AslamHenri Woelders
Jan 5, 2018·Ecology and Evolution·Takahiro KatoNobuyuki Kutsukake
May 31, 2013·Integrative and Comparative Biology·Vivian C Goerlich-JanssonTon G G Groothuis
Jul 28, 2017·Scientific Reports·Quanlin LiJiangxia Zheng
Dec 15, 2018·Journal of the Science of Food and Agriculture·Xiao-le XiangYon-Guo Jin