A reliable method for spectrophotometric determination of glycine betaine in cell suspension and other systems

Analytical Biochemistry
Ma Guadalupe Valadez-BustosAmalio Santacruz-Varela

Abstract

Glycine betaine is a quaternary ammonium compound that accumulates in a large variety of species in response to different types of stress. Glycine betaine counteracts adverse effects caused by abiotic factors, preventing the denaturation and inactivation of proteins. Thus, its determination is important, particularly for scientists focused on relating structural, biochemical, physiological, and/or molecular responses to plant water status. In the current work, we optimized the periodide technique for the determination of glycine betaine levels. This modification permitted large numbers of samples taken from a chlorophyllic cell line of the grass Bouteloua gracilis to be analyzed. Growth kinetics were assessed using the chlorophyllic suspension to determine glycine betaine levels in control (no stress) cells and cells osmotically stressed with 14 or 21% polyethylene glycol 8000. After glycine extraction, different wavelengths and reading times were evaluated in a spectrophotometer to determine the optimal quantification conditions for this osmolyte. Optimal results were obtained when readings were taken at a wavelength of 290 nm at 48 h after dissolving glycine betaine crystals in dichloroethane. We expect this modification to p...Continue Reading

References

Sep 24, 1982·Science·P H YanceyG N Somero
Nov 10, 1998·Plant Physiology·J JoyardR Douce
Feb 14, 2002·Plant, Cell & Environment·R. Serraj, T. R. Sinclair
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Serge N Timasheff
Nov 1, 1986·Plant Physiology·P WeigelA D Hanson
Apr 1, 1992·Plant Physiology·W L KoukkariF B Salisbury
Jun 8, 1984·Science·D Le RudulierR C Valentine
Jul 6, 2014·Breeding Science·Xuekun ZhangTakeshi Nishio

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Citations

Dec 20, 2018·International Journal of Molecular Sciences·Natalia OsmolovskayaLudger A Wessjohann
Jan 5, 2019·The Journal of Experimental Biology·Amanda A WiesenthalJan-Peter Hildebrandt
Sep 4, 2021·Ecotoxicology and Environmental Safety·Giovanna Camargo do CarmoHalley Caixeta Oliveira

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