A reliable screening protocol for thalassemia and hemoglobinopathies in pregnancy: an alternative approach to electronic blood cell counting

American Journal of Clinical Pathology
Kanokwan SanchaisuriyaFrank P Schelp

Abstract

Primary screening for thalassemia and hemoglobinopathies usually involves an accurate blood count using an expensive electronic blood cell counter A cheaper alternative method was tested by using a modified osmotic fragility (OF) test and a modified dichlorophenolindophenol (DCIP) test. Altogether 423 pregnant Thai women participated in this project. Hemoglobin patterns and globin genotypes were determined using an automated high-performance liquid chromatography analyzer and polymerase chain reaction analysis of alpha- and beta-globin genes. Among the 423 subjects, 264 (62.4%) carried thalassemia genes. The combined OF and DCIP tests detected all pregnant carriers of the 3 clinically important thalassemias, ie, alpha0-thalassemia, beta-thalassemia, and hemoglobin E with a sensitivity of 100.0%, specificity of 87.1%, positive predictive value of 84.5%, and negative predictive value of 100.0%, which show more effectiveness than these values for the standard method based on RBC counts. A combination of modified OF and DCIP tests should prove useful and applicable to prenatal screening programs for thalassemia and hemoglobinopathies in communities with limited facilities and economic resources.

References

Mar 26, 1983·British Medical Journal·E Silvestroni, I Bianco
Aug 1, 1981·Journal of Medical Genetics·C KattamisS Pootrakul
Sep 1, 1994·European Journal of Haematology·Goonnapa Fucharoen, Supan Fucharoen
Jul 23, 1998·British Journal of Haematology

Citations

Mar 4, 2014·Blood Cells, Molecules & Diseases·Naruwat PakdeeSupan Fucharoen
Feb 9, 2017·PloS One·Ernesto Cortés-CastellVicente Francisco Gil-Guillén
Jun 8, 2014·Journal of Medical Screening·Patcharawadee PrayalawSupan Fucharoen
Apr 29, 2008·Genetic Testing·Roshan ColahDipika Mohanty
Jun 26, 2008·Annals of Hematology·Kanokwan SanchaisuriyaPattara Sanchaisuriya
Jun 18, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Supada KhonyoungKate Grudpan
Mar 28, 2006·Annals of Hematology·Goonnapa FucharoenSupan Fucharoen
Jan 25, 2019·Journal of Pediatric Hematology/oncology·Mohsen MirzakhaniMohammad R Maracy
Jul 14, 2009·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Supatra SirichotiyakulTheera Tongsong
Nov 28, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Sakorn PornprasertKallayanee Treesuwan
Dec 17, 2009·Hemoglobin·Amal El-Beshlawy, Ilham Youssry
Aug 27, 2019·Frontiers in Molecular Biosciences·Angela AllenAnuja Premawardhena
Jul 11, 2020·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Prakas Kumar MandalTuphan Kanti Dolai
Nov 30, 2005·Prenatal Diagnosis

Related Concepts

Complete Blood Count
Electronics, Medical
Mean Corpuscular Hemoglobin Concentration Determination
Hemoglobinopathies
Osmotic Fragility Test
Pregnancy Complications, Hematologic
Thalassemia

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.