PMID: 9525583Apr 3, 1998Paper

A replication-competent retrovirus arising from a split-function packaging cell line was generated by recombination events between the vector, one of the packaging constructs, and endogenous retroviral sequences

Journal of Virology
H ChongR G Vile

Abstract

Previously we reported the presence of a replication-competent retrovirus in supernatant from a vector-producing line derived from a widely used split-function amphotropic packaging cell line. Rigorous routine screening of all retroviral stocks produced in our laboratory has not, previously or since, indicated the presence of such a virus. Replication-competent retroviruses have never previously been used in our laboratory, and stringent screening of all routinely used cell lines has not revealed the presence of any helper viruses. Therefore, it is highly unlikely that this virus represents an adventitious cross-contaminant or had been imported unknowingly with our cell line stocks. PCR studies with DNA from infected cell lines and Northern blot analysis and reverse transcriptase PCR with RNA from infected cells suggest that the helper virus arose by recombination events, at sites of partial homology, between sequences in the vector, one of the packaging constructs, and endogenous retroviral elements. These recombinations were not present in stocks of the packaging cell line or in an initial stock of the vector-producing line, indicating that these events occurred while the vector-producing line was being passaged for harvest o...Continue Reading

References

Oct 1, 1992·The Journal of Experimental Medicine·R E DonahueR Moen
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Citations

Jun 6, 2012·Scientific Reports·Ralf M LucheHans-Peter Kiem
Apr 8, 2000·Journal of Biomedical Science·J G Mikkelsen, F S Pedersen
Nov 29, 2002·Gene Therapy·J B Connolly
Apr 10, 2003·Journal of Medical Virology·Martin PlossFrank U Reuss
Jun 15, 2001·ILAR Journal·M B Dennis
Jun 7, 2005·Expert Opinion on Biological Therapy·Luisa BarzonGiorgio Palù
Jan 29, 2002·Human Gene Therapy·Chava Kimchi-SarfatyMichael M Gottesman
Apr 23, 2008·Expert Opinion on Drug Delivery·Chun Wang, Phuong-Truc Pham
Jan 1, 2014·BioResearch Open Access·Semih U TareenScott H Robbins
Oct 12, 2016·Viruses·Lizna M AliFarah Mustafa
Jan 29, 2020·International Journal of Biological Macromolecules·Porntipa PankongadisakHasan Uludağ
Aug 18, 2020·Cell Biochemistry and Biophysics·Zahra EsmaeilizadehZahra Fazeli
Jan 9, 1999·Journal of Virology·C Smerdou, P Liljeström
Oct 4, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kenneth CornettaCarl H June
Sep 14, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·P L SheridanS L Sauter
May 18, 2000·Reviews in Medical Virology·G PalùM Pizzato
Sep 14, 2018·Molecular Therapy. Methods & Clinical Development·Kenneth CornettaSteven A Rosenberg
Feb 8, 2003·Genomics·JunQing QianGregory M Shackleford
Jul 16, 2009·Retrovirology·Noura Salem Al DhaheriTahir A Rizvi
Sep 17, 2008·Journal of Biotechnology·Ana I AmaralPaula M Alves

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