PMID: 8970953Dec 1, 1996Paper

A replication function associated with the activation domain of the Epstein-Barr virus Zta transactivator

Journal of Virology
R T SariskyS D Hayward

Abstract

The Zta transactivator is crucial for both Epstein-Barr virus (EBV) lytic gene expression and lytic DNA replication. We have used a cotransfection-replication assay to examine the effect of mutations in the Zta activation domain (amino acids [aa] 1 to 167) on Zta replication activity. Deletion of Zta aa 25 to 86, which are critical for transcriptional activation of ori-Lyt, or aa 93 to 141 did not adversely affect replication of an ori-Lyt-containing target plasmid. However, removal of aa 2 to 25 (delta2-25) abolished replication activity. Within this subdomain, deletion of aa 2 to 10 (delta2-10) or mutation of codons 18 and 19 (m18/19) or 22 and 26 (m22/26) did not affect replication competency, while deletion of codons 13 to 19 (delta13-19) or mutation at codons 12 and 13 (m12/13) impaired Zta replication function. Each of the replication-negative Zta variants was capable of transactivating expression from both BHLF1 promoter-chloramphenicol acetyltransferase constructions and the BMRF1 promoter on endogenous EBV genomes in Raji cells with efficiency comparable to that of the wild-type polypeptide. Thus, a replication contribution of Zta was functionally separable from its transactivation activity and was supplied by the N-te...Continue Reading

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Citations

Oct 19, 2012·Journal of Virology·Ayman El-GuindyGeorge Miller
Jul 15, 2005·Journal of Virology·Yoshihiro IzumiyaHsing-Jien Kung
May 7, 2010·Journal of Virology·Andrew J RennekampPaul M Lieberman
May 28, 2005·Journal of Virology·Amy L Adamson
Oct 14, 2004·Journal of Virology·Yuling LiShannon Kenney
May 24, 2007·International Journal of Cancer. Journal International Du Cancer·Richard J JonesShannon C Kenney
Dec 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Coral K WilleShannon C Kenney
Jan 1, 1997·Annual Review of Genetics·J Flint, T Shenk
Dec 13, 2002·Journal of Virology·Richard J KrausJanet E Mertz
Feb 27, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guan-Ting LiuChung-Pei Lee
Jun 11, 2020·Cancers·Diego GerminiYegor Vassetzky

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