PMID: 6973397Jul 1, 1981Paper

A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras

Cell and Tissue Kinetics
S V Hunt, M H Fowler

Abstract

The repopulation of the peripheral lymphoid compartment of lethally-irradiated rats reconstituted with lymphopoietic stem cells was studied. Cell lineages were traced by using genetic markers of cell surface molecules: immunoglobulin allotype for B lymphocytes and peripheral T cell alloantigen for T lymphocytes. Provided the markers had been bred on to a genetic background congenic with the hosts, they conferred neither an advantage nor disadvantage in competition with unmarked cells. The degree of chimaerism measured the lymphopoietic activity of the restorative inoculum. The most potent activity was found in foetal liver and spleen; next was infant spleen and bone marrow; then young adult bone marrow. Peripheral lymphoid tissues showed very little activity and thymus cells were inert. This tissue distribution, the stability of the chimaerism and the substantial expansion of numbers from the injected cells all point to the assay measuring an early stem cell. The overlap of subpopulations of lymphocytes in the rat thoracic duct was studied. A method for the conjugation of fluorescein to antibodies while they are attached to immuno-adsorbent affinity columns is also described.

References

May 1, 1976·The Journal of Experimental Medicine·J L Kadish, R S Basch
Jan 1, 1976·Contemporary Topics in Molecular Immunology·R Nezlin, O Rokhlin
Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·J J OwenH N Willcox
Jun 1, 1979·European Journal of Immunology·W R McMaster, A F Williams
Sep 19, 1979·The Journal of Experimental Medicine·Y KitamuraM Seki
Jun 1, 1979·Developmental Biology·Y KitamuraS Go
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G A GutmanL Hood
Jan 1, 1976·Biochemical Society Transactions·S V Hunt, E Duvall
Jun 1, 1971·Immunochemistry·O V RokhlinR S Nezlin
Jul 19, 1966·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H S MicklemJ Gray
Apr 1, 1973·Cellular Immunology·J Sprent, A Basten
Mar 1, 1974·The Journal of Experimental Medicine·S V Hunt, A F Williams
Feb 1, 1974·European Journal of Immunology·J C Jensenius, A F Williams
Jun 1, 1972·The Journal of Experimental Medicine·L LafleurR A Phillips
Apr 1, 1972·Journal of Cellular Physiology·H S MicklemD S Papworth
Aug 1, 1980·European Journal of Immunology·R J BrideauA F Williams
Apr 1, 1980·The Biochemical Journal·D W Mason, A F Williams
Jan 1, 1971·European Journal of Immunology·D Armerding

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Citations

Jan 1, 1994·European Journal of Applied Physiology and Occupational Physiology·P O IversenH B Benestad
Oct 1, 1987·The Histochemical Journal·F Colbatzky, W Hermanns
Aug 28, 1990·Journal of Immunological Methods·P L GoossensG Milon
Oct 1, 1992·Veterinary Immunology and Immunopathology·C J WoolvertonR B Sartor
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G BertonS Gordon
Jun 1, 1983·The Journal of Experimental Medicine·C W PughH W Steer
Jul 1, 1985·The Journal of Experimental Medicine·W A JefferiesA F Williams
Nov 1, 1985·The Journal of Experimental Medicine·J Green, R Jotte
Mar 1, 1988·The Journal of Experimental Medicine·D Gray
Jun 1, 1989·The Journal of Experimental Medicine·E M BoltonJ A Bradley
Dec 1, 1990·The Journal of Experimental Medicine·J A GracieJ A Bradley
Nov 1, 1991·The Journal of Experimental Medicine·D Gray, P Matzinger
May 1, 1992·The Journal of Experimental Medicine·M H HermansD Opstelten
Nov 1, 1993·The Journal of Experimental Medicine·M BrebanJ D Taurog
Nov 1, 1996·The Journal of Experimental Medicine·A L FordJ D Sedgwick
Nov 17, 1998·The Journal of Experimental Medicine·M J PennyB M Hall
Mar 2, 1999·The Journal of Experimental Medicine·B Seddon, D Mason
Aug 3, 1999·The Journal of Experimental Medicine·M K WildP A van der Merwe
Jun 5, 2003·The Journal of Experimental Medicine·Ingunn H WestgaardSigbjorn Fossum
Dec 10, 1998·Transplantation·B C JaquesJ A Bradley

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