A report of nonexistence of the non-Helicobacter pylori Helicobacter species in Iranian patients suffering from inflammatory bowel disease.

Folia Microbiologica
Samira PirmaneshMohammad Reza Zali

Abstract

Inflammatory bowel disease is a chronic, relapsing-remitting gastrointestinal disorder which has become a serious global concern, and it imposes a great degree of health and economic burdens on communities worldwide. Although the presence of non-Helicobacter pylori Helicobacter (NHPH) microorganisms has been reported in various gastrointestinal disorders, their putative role in the pathogenesis of IBD has been a matter of controversy. The present study aimed to investigate the existence of gastric and enterohepatic NHPHs and their probable coinfection with H. pylori in IBD. Totally, 168 clinical specimens including 70 colonic biopsies and 98 fecal specimens were obtained from IBD patients. Genomic DNA was extracted from all samples, and its quality and concentration were assessed by β-globin PCR and spectrophotometry. The Helicobacter genus-specific PCR was performed using 16S rRNA gene. All samples were also tested for H. pylori infection by PCR of ureC gene fragment (glmM). The presence of NHPH was examined by using species-specific PCR assays. Based on PCR results, H. pylori was detected in 12.9% and 3.1% of colonic biopsies and fecal specimens, respectively. However, no statistically significant correlation was observed (P ...Continue Reading

References

Jun 1, 1994·PCR Methods and Applications·C E GreerM M Manos
Oct 16, 2001·Gut·J L O'RourkeA Lee
Jun 29, 2002·Journal of Clinical Microbiology·James G FoxJohn M Cullen
Sep 3, 2003·Alimentary Pharmacology & Therapeutics·S J BellM A Kamm
Feb 10, 2004·Journal of Clinical Microbiology·Martin GrehanHazel Mitchell
Jun 9, 2004·Journal of Clinical Microbiology·Ulrich R M BohrPeter Malfertheiner
Mar 9, 2005·Veterinary Research Communications·K Van den BulckA Decostere
Apr 9, 2005·Journal of Clinical Microbiology·Farhana KauserNiyaz Ahmed
Sep 27, 2005·Helicobacter·Dominic De GrooteRichard Ducatelle
Jun 8, 2006·Journal of Clinical Microbiology·Li ZhangHazel Mitchell
Mar 10, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·U R M BohrP Malfertheiner
Oct 20, 2010·FEMS Immunology and Medical Microbiology·Richard HansenGeorgina L Hold
Apr 11, 2012·Alimentary Pharmacology & Therapeutics·R M Genta, A Sonnenberg
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Isabelle C ArnoldAnne Müller
May 10, 2013·Journal of Clinical Microbiology·Emiko RimbaraKeigo Shibayama
Feb 5, 2014·World Journal of Microbiology & Biotechnology·Abbas YadegarMohammad Reza Zali
May 24, 2014·World Journal of Microbiology & Biotechnology·Abbas YadegarMohammad Reza Zali
Jun 11, 2014·World Journal of Gastroenterology : WJG·Konstantinos PapamichaelGerassimos J Mantzaris
Nov 8, 2014·Alimentary Pharmacology & Therapeutics·R M Genta, A Sonnenberg
Nov 28, 2014·World Journal of Gastrointestinal Surgery·Amosy E M'Koma
Apr 29, 2015·World Journal of Gastroenterology : WJG·Xiao-Wei WuFang-Yu Wang
Jan 23, 2017·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Seiji ShiotaHashem B El-Serag
Feb 28, 2017·Intestinal research·Prashant SinghVineet Ahuja
Oct 16, 2018·Frontiers in Microbiology·Tao Zuo, Siew C Ng
Feb 16, 2019·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Johan Burisch, Tine Jess
Aug 6, 2019·Frontiers in Microbiology·Alessandra Lo PrestiMichele Pier Luca Guarino

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