A Reporter System for Translational Readthrough of Stop Codons in Human Cells.

FEBS Open Bio
Patrick J HalveyRobbert J C Slebos

Abstract

Agents to induce readthrough of premature termination codons (PTCs) are useful research tools and potential therapeutics. Reporters used to detect PTC readthrough are gene-specific and thus are not suited to for general assessment of readthrough activity or in cases where PTC-inactivated genes are unknown. Here we describe a GFP-based reporter construct pMHG-W57* which is capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry. pMHG-W57* may be used as a general indicator of PTC readthrough in living cells and obviates the need for gene-specific recoding sequences in reporter constructs.

References

Aug 17, 1999·The Journal of Clinical Investigation·E R Barton-DavisH L Sweeney
Oct 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chih-Hung LaiRichard A Gatti
Jul 30, 2005·Blood·Paula D JamesDavid Lillicrap
Apr 24, 2007·Nature·Ellen M WelchH Lee Sweeney

❮ Previous
Next ❯

Citations

Aug 8, 2015·Anais Da Academia Brasileira De Ciências·Renata V VelhoIda V D Schwartz
Dec 2, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Amnon WittensteinRina Rosin-Arbesfeld
Nov 21, 2019·The British Journal of Dermatology·A PeledE Sprecher
Jun 21, 2020·Biomolecules·Kseniya A LashkevichSergey E Dmitriev

❮ Previous
Next ❯

Methods Mentioned

BETA
phosphotransferase
transfections
transfection
fluorescence microscopy
fluorescence
flow cytometry

Software Mentioned

FACSDiva

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Nature Genetics
Dan Mercola, John Welsh
Proceedings of the National Academy of Sciences of the United States of America
Chunmei YangSteve S Sommer
Journal of Thrombosis and Haemostasis : JTH
M PinottiInternational Factor VII Deficiency Study Group
© 2021 Meta ULC. All rights reserved