A repressible female-specific lethal genetic system for making transgenic insect strains suitable for a sterile-release program

Proceedings of the National Academy of Sciences of the United States of America
J C Heinrich, M J Scott


We have developed a tetracycline-repressible female-specific lethal genetic system in the vinegar fly Drosophila melanogaster. One component of the system is the tetracycline-controlled transactivator gene under the control of the fat body and female-specific transcription enhancer from the yolk protein 1 gene. The other component consists of the proapoptotic gene hid under the control of a tetracycline-responsive element. Males and females of a strain carrying both components are viable on medium supplemented with tetracycline, but only males survive on normal medium. A strain with such properties would be ideal for a sterile-insect release program, which is most effective when only males are released in the field.


Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Jan 11, 1991·Cell·A Varshavsky
Oct 22, 1982·Science·G M Rubin, A C Spradling
Jul 22, 1995·Molecular & General Genetics : MGG·L SøndergaardM Bownes
Apr 1, 1997·Current Opinion in Genetics & Development·P K Geyer
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·L ZhouJ R Nambu
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·Nijole JasinskieneFrank H Collins
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·C J CoatesAnthony A James
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·Alfred M HandlerS H Saul
Aug 6, 1998·Insect Biochemistry and Molecular Biology·T W Sappington, A S Raikhel
Oct 13, 1998·Scientific American·V C Jordan
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·W N HainingH Steller
Oct 1, 1960·Scientific American·E F KNIPLING
Feb 1, 1994·Genome Génome / Conseil National De Recherches Canada·Gerald FranzP Kerremans
Oct 1, 1997·Development Genes and Evolution·C Tortiglione, M Bownes


Jun 2, 2011·Apoptosis : an International Journal on Programmed Cell Death·Marc F ScheteligAlfred M Handler
Apr 14, 2010·Transgenic Research·Holly J FergusonMalcolm Fraser
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Maria MarkakiC Savakis
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Kathryn A RaphaelMarianne Frommer
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Katia KomitopoulouAnastassios C Mintzas
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Alan S RobinsonP W Atkinson
Nov 19, 2013·Insect Biochemistry and Molecular Biology·Xiu-Ye WangYong-Zhen Xu
May 30, 2002·Molecular and Biochemical Parasitology·Luke Alphey, Morten H Andreasen
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Carsten HornErnst A Wimmer
Sep 13, 2002·Insect Biochemistry and Molecular Biology·P W Atkinson
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Luke Alphey
Jun 8, 2002·Mutation Research·Alan S Robinson
Aug 7, 2003·Trends in Parasitology·Mark Q Benedict, Alan S Robinson
Feb 27, 2007·Nature Biotechnology·Guoliang FuLuke Alphey
Dec 17, 2002·Nature Biotechnology·Carsten Horn, Ernst A Wimmer
Mar 1, 2003·Nature Reviews. Genetics·Ernst A Wimmer
Nov 28, 2001·European Journal of Biochemistry·S O ZakharkinH Beneš
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Guoliang FuLuke Alphey
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anjiang TanYongping Huang
Sep 4, 2009·Vector Borne and Zoonotic Diseases·Luke AlpheyStephen L Dobson
Sep 18, 2004·Insect Molecular Biology·D MuñozAnthony A James
Dec 4, 2003·Annual Review of Entomology·Fred Gould, Paul Schliekelman
Jun 17, 2006·BMC Biotechnology·Abhimanyu SarkarMaxwell J Scott
Dec 16, 2009·Malaria Journal·Alan S RobinsonJorge Hendrichs
Dec 16, 2009·Malaria Journal·Philippos Aris PapathanosAndrea Crisanti
Dec 16, 2009·Malaria Journal·Flaminia CatterucciaErnst A Wimmer
Mar 22, 2007·BMC Biology·Hoang Kim PhucLuke Alphey
Feb 10, 2012·PLoS Neglected Tropical Diseases·R Guy ReevesFloyd A Reed
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R RyuD N Arnosti
Aug 15, 2014·Proceedings. Biological Sciences·Philip T LeftwichLuke Alphey
Feb 14, 2002·Insect Molecular Biology·J C HeinrichMaxwell J Scott
Dec 5, 2014·BMC Genetics·Efthimia SagriKostas D Mathiopoulos
Jan 1, 2011·Journal of Insect Science·Alicia L BassoLuis A Quesada-Allué
Sep 30, 2010·Journal of Genetics·Tarig Dafa'allaLuke Alphey
Oct 3, 2001·Journal of Medical Entomology·M L AllenC S Levesque
Jan 2, 2016·Biochemical and Biophysical Research Communications·Fang Li, Maxwell J Scott
Apr 19, 2016·Evolutionary Applications·Philip T LeftwichTracey Chapman
Nov 10, 2012·Insect Biochemistry and Molecular Biology·Christian E OgaugwuErnst A Wimmer
Aug 4, 2012·Insect Biochemistry and Molecular Biology·Marc F Schetelig, Alfred M Handler
Jun 11, 2011·Trends in Parasitology·William C BlackAnthony A James
Oct 6, 2007·Insect Biochemistry and Molecular Biology·Kirsty C CondonLuke Alphey
Feb 14, 2007·Insect Biochemistry and Molecular Biology·Ivana Viktorinová, Ernst A Wimmer
Apr 2, 2009·Medical and Veterinary Entomology·Alfred M HandlerSteven R Skoda
Nov 15, 2007·Evolution; International Journal of Organic Evolution·Fred Gould
Mar 27, 2010·Insect Molecular Biology·D S YamamotoS Yoshida
Dec 19, 2009·Insect Molecular Biology·J WangY Park
Aug 25, 2004·Trends in Parasitology·Steven P Sinkins, Ian M Hastings
Jun 15, 2014·Insect Biochemistry and Molecular Biology·Fang LiMaxwell J Scott
Jun 25, 2015·Parasites & Vectors·André Barreto Bruno Wilke, Mauro Toledo Marrelli
Apr 19, 2015·Malaria Journal·James E GentileGregory R Madey
Sep 17, 2013·Insect Biochemistry and Molecular Biology·Sanyuan MaQingyou Xia
Jan 2, 2007·Toxicon : Official Journal of the International Society on Toxinology·Glenn F King
Nov 11, 2008·Cell Host & Microbe·Olle TereniusAnthony A James
Sep 23, 2014·International Journal for Parasitology·R M SandemanV M Bowles
Nov 7, 2016·Journal of Theoretical Biology·Benjamin Watkinson-Powell, Nina Alphey
Nov 6, 2015·Scientific Reports·Ying Yan, Maxwell J Scott
Sep 18, 2012·Revista Do Instituto De Medicina Tropical De São Paulo·André Barreto Bruno Wilke, Mauro Toledo Marrelli
Dec 18, 2019·Archives of Insect Biochemistry and Physiology·Konstantina T TsoumaniPhilippos Aris Papathanos
Feb 25, 2020·Archives of Insect Biochemistry and Physiology·Maria-Eleni Gregoriou, Kostas D Mathiopoulos
Feb 6, 2020·G3 : Genes - Genomes - Genetics·Katherine E KnudsenMaxwell J Scott
Aug 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zhongjie ZhangYongping Huang
Jun 18, 2017·Biological Invasions·Tim Harvey-SamuelLuke Alphey
Jun 26, 2018·Agricultural and Forest Entomology·Nina Alphey, Michael B Bonsall
Dec 26, 2018·Parasites & Vectors·Elzbieta Krzywinska, Jaroslaw Krzywinski
Oct 14, 2017·Nature Communications·Maciej MaselkoMichael J Smanski
May 17, 2017·Cold Spring Harbor Perspectives in Medicine·Neil F LoboFrank H Collins
May 20, 2018·Nature Reviews. Microbiology·Heather A Flores, Scott L O'Neill
Sep 2, 2009·Revista de saúde pública·André Barreto Bruno WilkeMauro Toledo Marrelli
Jun 20, 2020·Nature Communications·Yang ZhaoAlfred M Handler
Sep 26, 2012·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Antoinette TordesillasRobert P Behringer

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