A requirement for DICER to maintain full promoter CpG island hypermethylation in human cancer cells.

Cancer Research
Angela H TingStephen B Baylin

Abstract

Promoter hypermethylation is a prevalent phenomenon, found in virtually all cancer types studied thus far, and accounts for tumor suppressor gene silencing in the absence of genetic mutations. The mechanism behind the establishment and maintenance of such aberrant hypermethylation has been under intense study. Here, we have uncovered a link between aberrant gene silencing associated with promoter CpG island DNA methylation and the siRNA/miRNA processing enzyme, DICER, in human cancer cells. By comparing demethylated HCT116 colon cancer cells with HCT116 cells genetically rendered hypomorphic for DICER, we identified a group of epigenetically silenced genes that became reactivated in the absence of functional DICER. This reactivation is associated with a dramatic loss of localized promoter DNA hypermethylation. Thus, intact DICER is required to maintain full promoter DNA hypermethylation of select epigenetically silenced loci in human cancer cells.

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M FrommerC L Paul
Feb 11, 1994·Cell·M WasseneggerH L Sänger
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Daiya Takai, Peter A Jones
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Jul 18, 2003·Current Biology : CB·Gurumurthy D ShankaranarayanaShiv I S Grewal
Oct 7, 2003·Nature Genetics·Emily BernsteinGregory J Hannon
May 18, 2004·Current Opinion in Cell Biology·Shiv I S Grewal, Judd C Rice
Aug 7, 2004·Science·Kevin V MorrisDavid J Looney
Jun 21, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Daniela CastanottoJohn J Rossi
Oct 19, 2005·The Journal of Biological Chemistry·Byron H LeeWilliam G Nelson
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jordan M CumminsVictor E Velculescu
Dec 8, 2006·Nucleic Acids Symposium Series·Bethany A Janowski, David R Corey
Dec 13, 2006·Genes & Development·Angela H TingStephen B Baylin
Sep 26, 2007·PLoS Genetics·Kornel E SchuebelStephen B Baylin

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Citations

Oct 31, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Lilach M Friedman, Karen B Avraham
Jan 16, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Pirkko Muhonen, Harry Holthofer
Jun 11, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael T McCabePaula M Vertino
Feb 24, 2009·BMC Molecular Biology·Yuliang TanRunsheng Chen
Mar 23, 2011·Molecular Cancer·Tony Bou KheirLennart Friis-Hansen
Nov 8, 2013·Functional & Integrative Genomics·Sadegh Azimzadeh JamalkandiAli Masoudi-Nejad
Nov 10, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Randall S Gieni, Michael J Hendzel
Jul 2, 2010·Pharmaceuticals·Paul M HowellHung T Khong
Oct 7, 2008·The International Journal of Biochemistry & Cell Biology·Sònia Guil, Manel Esteller
Sep 10, 2008·Trends in Genetics : TIG·Anna Kloc, Robert Martienssen
Jan 8, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nicholas J HandJoshua R Friedman
Aug 21, 2012·Molecular Oncology·Hiromu SuzukiMasahiro Kai
Mar 15, 2015·Acta histochemica·Rosario GulinoMassimo Gulisano
Oct 24, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Long-Cheng Li
Aug 28, 2012·RNA Biology·Lei ChangGuangming Zhou
Jan 22, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Angeline A Giangreco, Larisa Nonn
Feb 25, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C FaberT Kirchner
May 16, 2012·The International Journal of Biochemistry & Cell Biology·Antonios N GargalionisAthanasios G Papavassiliou
Dec 10, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Nicholas C HoffendThomas B Tomasi
Jan 12, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Manon van EngelandJames G Herman
Nov 10, 2010·Nature Reviews. Urology·Antoinette S PerryDonal Hollywood
Jun 17, 2015·Oncotarget·Xin Yu, Zheng Li
Oct 9, 2019·Journal of Experimental & Clinical Cancer Research : CR·Yongqiang QiYongjun Chen

❮ Previous
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