A requirement for dimerization of HP1Hsalpha in suppression of breast cancer invasion

The Journal of Biological Chemistry
Laura E NorwoodLori L Wallrath

Abstract

The development and progression of cancer is controlled by gene expression, often regulated through chromatin packaging. Heterochromatin protein 1(Hsalpha) (HP1(Hsalpha)), one of three human HP1 family members, participates in heterochromatin formation and gene regulation. HP1(Hsalpha) possesses an amino-terminal chromodomain, which binds methylated lysine 9 of histone H3 (meK9 H3), and a carboxyl-terminal chromoshadow domain (CSD) that is required for dimerization and interaction with partner proteins. HP1(Hsalpha) is down-regulated in invasive metastatic breast cancer cells compared with poorly invasive nonmetastatic breast cancer cells. Expression of EGFP-HP1(Hsalpha) in highly invasive MDA-MB-231 cells causes a reduction in in vitro invasion, without affecting cell growth. Conversely, knock-down of HP1(Hsalpha) levels in the poorly invasive breast cancer cell line MCF-7 increased invasion, without affecting cell growth. To determine whether functions of the CSD were required for the regulation of invasion, mutant forms of HP1(Hsalpha) were expressed in MDA-MB-231 cells. A W174A mutation that disrupts interactions between HP1(Hsalpha) and PXVXL-containing partner proteins reduced invasion similar to that of the wild type pro...Continue Reading

References

Sep 15, 1990·Cancer·J E Price
Jan 1, 1995·Annual Review of Genetics·K S Weiler, B T Wakimoto
Sep 11, 1999·Breast Cancer Research and Treatment·D A KirschmannM J Hendrix
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Nov 4, 2000·Journal of the National Cancer Institute·B A YoshidaC W Rinker-Schaeffer
Sep 22, 2001·The EMBO Journal·S A JacobsS Khorasanizadeh
Oct 13, 2001·Science·P BernardR C Allshire
Jan 10, 2002·Nature Cell Biology·Nobuhiro NonakaYoshinori Watanabe
Mar 20, 2002·Nature Reviews. Cancer·A Balmain
Mar 22, 2002·Nature Reviews. Cancer·A G Knudson
Apr 18, 2002·Genes & Development·Patrick J PaddisonDouglas S Conklin
May 16, 2002·Journal of Mammary Gland Biology and Neoplasia·D F HayesV Stearns
May 16, 2002·Journal of Mammary Gland Biology and Neoplasia·M T Debies, D R Welch
Aug 2, 2002·Cancer Cell·Patrick J Paddison, Gregory J Hannon
Aug 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuhong LiLori L Wallrath
Oct 11, 2002·Nature·Sooryanarayana VaramballyArul M Chinnaiyan
Feb 5, 2003·Biochemical and Biophysical Research Communications·Ken Yamamoto, Miki Sonoda
May 15, 2003·Clinical Breast Cancer·Patricia S SteegMassimiliano Salerno
Dec 4, 2003·Current Opinion in Cell Biology·Jean Paul Thiery
Mar 18, 2004·Journal of the National Cancer Institute·Patricia S Steeg
Jul 1, 2004·Gene·Laura E NorwoodLori L Wallrath
Aug 3, 2004·Nature Medicine·Bert Vogelstein, Kenneth W Kinzler
Sep 11, 2004·Carcinogenesis·Chand Khanna, Kent Hunter
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Wenjun Guo, Filippo G Giancotti
Oct 28, 2004·Journal of Leukocyte Biology·Emilie LukásováIva Kroupová
Feb 11, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Diane E CrydermanLori L Wallrath

❮ Previous
Next ❯

Citations

Jul 6, 2007·Nature Reviews. Cancer·Gayle J PageauJeanne B Lawrence
Sep 7, 2012·Nature Reviews. Cancer·Gabriele SulliFabrizio d'Adda di Fagagna
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tasneem Bawa-KhalfeEdward T H Yeh
Jul 2, 2010·Cold Spring Harbor Perspectives in Biology·Tom Misteli
Jul 2, 2009·Breast Cancer Research : BCR·Jason G LieberthalNaoko Tanese
Mar 29, 2013·Journal of Molecular Endocrinology·Momoe ItsumiSeiji Naito
May 21, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maria S TretiakovaTracy K Hale
Jan 6, 2010·EMBO Molecular Medicine·Leanne De KoningGeneviève Almouzni
Jun 19, 2012·Clinical Epigenetics·Robert TellRichard V Benya
Jun 4, 2011·Cell Biology International·Alžběta JugováEva Bártová
Oct 23, 2016·Cellular and Molecular Life Sciences : CMLS·Lidong Sun, Jia Fang
Oct 18, 2008·Mutation Research·George K DialynasLori L Wallrath
Oct 19, 2010·Biochimica Et Biophysica Acta·Radhika A Varier, H T Marc Timmers
Feb 20, 2007·Mutation Research·Timothy J Moss, Lori L Wallrath
Jan 15, 2015·Cancer Biology & Therapy·Johan Vad-Nielsen, Anders Lade Nielsen
Jun 14, 2017·Nucleic Acids Research·Stefan J Tekel, Karmella A Haynes
Apr 26, 2008·The Journal of Urology·Ellen ShapiroSusan K Logan
Feb 27, 2019·Archives of Pharmacal Research·Jong Woo Park, Jeung-Whan Han
Mar 5, 2011·Molecular Carcinogenesis·Rune ThomsenAnders L Nielsen
Aug 14, 2019·Scientific Reports·Andre C LoyolaWillis X Li
Sep 10, 2014·International Journal of Oncology·Sang Ah YiSo Hee Kwon
Jan 8, 2021·Cancer Investigation·Amit SharmaIngo G H Schmidt-Wolf
Mar 4, 2014·Physical Review Letters·Hang ZhangJianhua Xing
Feb 2, 2011·The American Journal of Pathology·Robert TellRichard V Benya
Dec 10, 2009·Journal of Chemical Information and Modeling·Fergal P CaseyDenis C Shields
Jun 5, 2021·Biophysical Journal·Susav PradhanMartin A K Williams
Mar 29, 2008·Current Opinion in Genetics & Development·Jamy C Peng, Gary H Karpen
Oct 14, 2008·Trends in Cell Biology·Willis X Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.